ANALYTICAL ULTRACENTRIFUGE PURCHASE: MOLECULAR GENETICS

分析超速离心机购买：分子遗传学

• 批准号: 7335165
• 负责人: ROBERT S HODGES
• 金额: $ 26.05万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335165
• 关键词: Coronaviridae; RNA; protein protein interaction; proteins; schools

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The University of Colorado School of Medicine will purchase an analytical ultracentrifugation instrument with support from the NCRR Shared Instrumentation Grant Program. This instrument is required to meet the growing demand for the accurate and rapid determination of size, shape, association and interactions of macromolecules and complexes. The increased demand results from expansion of the faculty in several departments in the School as well as the establishment of the interdepartmental Program in Biomolecular Structure. The requested instrument will greatly improve the existing resources and will accelerate research in a broad range of systems, including: Analysis of self-association and heteroprotein interactions in the human progesterone receptor (D.L. Bain) Obtaining a mechanistic understanding of viral DNA packaging (C.E. Catalano). De novo design of coiled-coils to understand protein folding and stability and investigations of coiled- coils in native proteins, e.g., motor proteins, transporter of ProP and SARS-coronavirus (R.S. Hodges). Determining functional differences of isoforms of progesterone (K. Horwitz). Examining folding of biologically important RNA molecules including functional RNAs from poliovirus and related viruses, and studying protein-RNA interactions from foot and mouth disease virus (J.S. Kieft). Analysis of protein assembly mechanisms and quantification of strength of protein-protein interactions in cellular replicases (C. McHenry). Other areas of current research also include determination of oligomerization and/or protein-protein interactions in a range of receptors (e.g., B cell complement receptor, glycoproteins of coronavirus, ligand binding proteins of estrogen receptor); the role of ETS transcription factors in epithelial cell development and tumorigenesis; subcellular organization of signal transduction pathways; lymphocyte-specific transcription by early B cell factor; storage and intracellular trafficking of lipids; characterization of tissue plasminogen activator protein; characterization of genetically identified telomerase and end-protection complexes in yeast; and molecular mechanisms of pre-mRNA splicing.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：科罗拉多大学医学院将在NCRR共享仪器资助计划的支持下购买一台分析超离心仪器。这种仪器需要满足日益增长的需求，准确和快速测定的大小，形状，协会和相互作用的大分子和复合物。需求的增加是由于学校几个部门的教师队伍的扩大以及生物分子结构跨部门计划的建立。所要求的工具将大大改善现有资源，并将加速在广泛的系统中进行研究，包括： 分析人孕酮受体中的自缔合和杂蛋白相互作用（D.L.贝恩） 获得对病毒DNA包装的机械理解（C.E. Catalano）。 重新设计卷曲螺旋以了解蛋白质折叠和稳定性，并研究天然蛋白质中的卷曲螺旋，例如，马达蛋白、ProP转运蛋白和SARS冠状病毒（R.S. Hodges）。确定孕酮（K。Horwitz）。 研究生物学上重要的RNA分子的折叠，包括脊髓灰质炎病毒和相关病毒的功能性RNA，并研究口蹄疫病毒的蛋白质-RNA相互作用（J.S. Kieft）。 蛋白质组装机制的分析和细胞复制酶中蛋白质-蛋白质相互作用强度的定量（C。麦克亨利）。目前研究的其他领域还包括确定一系列受体中的寡聚化和/或蛋白质-蛋白质相互作用（例如，B细胞补体受体、冠状病毒的糖蛋白、雌激素受体的配体结合蛋白）; ETS转录因子在上皮细胞发育和肿瘤发生中的作用;信号转导途径的亚细胞组织;早期B细胞因子的淋巴细胞特异性转录;脂质的储存和细胞内运输;组织纤溶酶原激活蛋白的表征;酵母中遗传鉴定的端粒酶和末端保护复合物的表征;以及前体mRNA剪接的分子机制。