Analytical Ultracentrifuge Purchase

描述(由申请者提供)：科罗拉多大学医学院将在NCRR共享仪器资助计划的支持下购买一台分析超速离心仪。为了满足日益增长的对大分子和络合物的尺寸、形状、缔合和相互作用的准确和快速测定的需求，该仪器是必需的。需求增加的原因是学院几个系的师资队伍扩大，以及生物分子结构系间项目的建立。所要求的仪器将极大地改善现有资源，并将加快对各种系统的研究，包括： 人孕酮受体(D.L.Bain)的自结合和异蛋白相互作用分析 获得对病毒DNA包装的机械理解(C.E.卡塔拉诺) 螺旋线圈的从头设计以了解蛋白质折叠和稳定性，并研究天然蛋白质中的螺旋线圈，例如马达蛋白、Prop的转运体和SARS冠状病毒(R.S.Hodges)。孕酮亚型功能差异的测定(K.Horwitz)。 研究重要的生物RNA分子的折叠，包括来自脊髓灰质炎病毒和相关病毒的功能RNA，并研究来自口蹄疫病毒的蛋白质-RNA相互作用(J.S.Kieft)。 细胞复制酶中蛋白质组装机制的分析和蛋白质-蛋白质相互作用强度的量化(C.麦克亨利) 目前研究的其他领域还包括确定一系列受体(例如B细胞补体受体、冠状病毒的糖蛋白、雌激素受体的配体结合蛋白)中的寡聚和/或蛋白质之间的相互作用；ETS转录因子在上皮细胞发育和肿瘤发生中的作用；信号转导途径的亚细胞组织；早期B细胞因子的淋巴细胞特异性转录；脂类的储存和细胞内转运；组织纤溶酶原激活剂蛋白的特性；酵母中经基因鉴定的端粒酶和末端保护复合体的特性；以及Pre-mRNA剪接的分子机制。

DESCRIPTION (provided by applicant): The University of Colorado School of Medicine will purchase an analytical ultracentrifugation instrument with support from the NCRR Shared Instrumentation Grant Program. This instrument is required to meet the growing demand for the accurate and rapid determination of size, shape, association and interactions of macromolecules and complexes. The increased demand results from expansion of the faculty in several departments in the School as well as the establishment of the interdepartmental Program in Biomolecular Structure. The requested instrument will greatly improve the existing resources and will accelerate research in a broad range of systems, including:  Analysis of self-association and heteroprotein interactions in the human progesterone receptor (D.L. Bain)  Obtaining a mechanistic understanding of viral DNA packaging (C.E. Catalano).  De novo design of coiled-coils to understand protein folding and stability and investigations of coiled- coils in native proteins, e.g., motor proteins, transporter of ProP and SARS-coronavirus (R.S. Hodges).  Determining functional differences of isoforms of progesterone (K. Horwitz).  Examining folding of biologically important RNA molecules including functional RNAs from poliovirus and related viruses, and studying protein-RNA interactions from foot and mouth disease virus (J.S. Kieft).  Analysis of protein assembly mechanisms and quantification of strength of protein-protein interactions in cellular replicases (C. McHenry). Other areas of current research also include determination of oligomerization and/or protein-protein interactions in a range of receptors (e.g., B cell complement receptor, glycoproteins of coronavirus, ligand binding proteins of estrogen receptor); the role of ETS transcription factors in epithelial cell development and tumorigenesis; subcellular organization of signal transduction pathways; lymphocyte-specific transcription by early B cell factor; storage and intracellular trafficking of lipids; characterization of tissue plasminogen activator protein; characterization of genetically identified telomerase and end-protection complexes in yeast; and molecular mechanisms of pre-mRNA splicing.