OLYMPUS FLUOVIEW CONFOCAL LASER SCANNING MICROSCOPE

描述（由申请人提供）：申请资金用于购买奥林巴斯Fluoview FV500共聚焦激光扫描显微镜，该显微镜将集成到UCSB共享显微镜设施中。这个建立的设施已经为UCSB的研究人员提供了超过15年的核心显微设备和技术支持。拟议的共聚焦显微镜将延续共享显微镜设施的承诺，为UCSB研究界提供最先进的工具。所要求的仪器将取代已有7年历史的BioRad 1024显微镜，该显微镜目前受到可靠性差、可维修性降低和缺乏升级潜力的严重限制。新的显微镜在可用激光激发波长的数量、定量分析的能力和活细胞成像方面将有更大的能力。此外，新仪器在改进的计算硬件和软件方面具有优势，用于激光控制和图像捕获，更高的分辨率和更大的视场能力。赞助这项提案的11位首席研究员代表了五个不同的学术部门或研究单位，包括神经科学研究所、分子、细胞和发育生物学系、心理学系、化学和生物化学系以及生物分子科学与工程跨部门项目。每个人都有目前nih支持的研究项目，其中共聚焦显微镜是一个关键的研究组成部分。这些研究集中在生物医学问题上，包括神经退行性疾病，如阿尔茨海默病和帕金森病，眼部病理，如年龄相关性黄斑变性和视网膜脱离，癌症生物学和药理学，传染病的细胞和分子生物学，以及神经生理学和发育。共享显微镜设施中的共聚焦显微镜是UCSB校园内唯一可供这些研究人员使用的显微镜。目前的显微镜已经被广泛使用，在过去的五年里，产生的数据已经在40多篇同行评审的出版物中发表。由于拟议的仪器提供了越来越多的共聚焦研究应用，以及其精简的数据处理能力和优越的可靠性特点，预计这种生产力水平将继续扩大。增强的多重标记能力和支持活细胞研究的能力将允许研究人员探索我们当前仪器无法接近的新研究途径。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of an Olympus Fluoview FV500 confocal laser scanning microscope, which will be integrated into the UCSB Shared Microscopy Facility. This established facility has been providing core microscopic equipment and technical support to UCSB investigators for over 15 years. The proposed confocal microscope will perpetuate the commitment of the Shared Microscopy Facility to provide state-of-the art tools for the UCSB research community. The instrument requested will replace a seven-year-old BioRad 1024 microscope that is now seriously limited by poor reliability, decreasing repairability, and lack of potential for upgrade. The new microscope will have increased capabilities in terms of the number of available laser excitation wavelengths, the capacity for quantitative analyses, and the imaging of living cells. In addition, the new instrument affords advantages in terms of improved computational hardware and software for laser control and image capture, superior resolution, and larger field of view capabilities. The eleven principal investigators sponsoring this proposal represent five different academic departments or research units including the Neuroscience Research Institute, Department of Molecular, Cellular and Developmental Biology, Department of Psychology, Department of Chemistry and Biochemistry, and the Interdepartmental Program in Biomolecular Sciences and Engineering. Each has current NIH-supported research projects in which confocal microscopy is a critical investigational component. These investigations focus upon biomedical issues, including neuronal degenerative diseases such as Alzheimer's and Parkinson's, ocular pathologies such as age-related macular degeneration and retina] detachment, cancer biology and pharmacology, cell and molecular biology of infectious disease, as well as neuronal physiology and development. The confocal microscope in the Shared Microscopy Facility is the only one on the UCSB campus available to these investigators. The current microscope has been extensively utilized, generating data that has been presented in over 40 peer-reviewed publications in the last five years. It is anticipated that this level of productivity will continue to expand as a consequence of an increasing number of applications for confocal studies afforded by the proposed instrumentation, as well as its streamlined data handling capabilities and superior reliability characteristics. The enhanced multi-labeling capabilities and capacity to support living cell studies will allow investigators to explore novel research avenues that are unapproachable with our current instrumentation.