THE DEVELOPING AND ADULT VISUAL SYSTEM

该项目有几个具体目标，旨在增加我们的 对脊椎动物视网膜及其相互依赖的认识 视网膜色素上皮。这些研究将有助于我们的知识 视网膜组织和我们对视网膜反应的理解 视网膜受到损伤。光强增强对环锥体的影响 圆盘脱落将在地松鼠身上进行研究。突触组织 和人类视网膜的电路将分成连续的部分进行研究 电子显微镜，并对人视网膜神经细胞的形态进行了研究 高尔基体受孕技术。我们将使用光学和电子显微镜 放射自显影用于确定各种分子的摄取模式 (尿苷、脯氨酸、半乳糖、多巴胺、γ-氨基丁酸、麝香酚、 牛磺酸)对正常猫视网膜和视网膜分离所致的损伤 神经视网膜和色素上皮。对这些东西的理解的变化 受伤后分子随时间的变化将被监测以确定我们是否可以 查找与所描述的形态变化平行的代谢变化 我们和其他人。细胞骨架蛋白(微管蛋白、 微管相关蛋白，肌动蛋白，神经细丝，波形蛋白，胶质细胞 纤维酸性蛋白)和表面糖将在 正常视网膜和分离的视网膜细胞的细胞化学研究 抗体和凝集素。细胞骨架分布的变化 蛋白质也将在分离神经视网膜和色素后进行研究。 并与先前描述的形态变化相关联。 我们将使用间接荧光来检测凝集素结合，间接 免疫荧光、过氧化物酶-抗-过氧化物酶、蛋白A-金或 免疫金技术将被用于研究抗体结合。蛋白 将使用A-金或免疫金技术来研究抗体结合 电子显微镜。将会产生抗猫的单抗。 视网膜和色素上皮及其特异性的定位 免疫细胞化学光镜和电子显微镜检测抗原。 该项目最重要的长期目标之一是使用 从这些实验中获得的信息可以挽救视网膜细胞 视网膜损伤。

This project has several specific goals aimed at increasing our understanding of the vertebrate retina and its interdependence on the retinal pigment epithelium. These studies will contribute to our knowledge of retinal organization and to our understanding of the responses of the retina to injury. The effect of increased light intensity on cyclic cone disc shedding will be studied in ground squirrels. Synaptic organization and circuitry of the human retina will be studied in serial sections by electron microscopy, and the morphology of human retinal neurons studied by Golgi impregnation techniques. We will use light and electron microscope autoradiography to determine the patterns of uptake of various molecules (uridine, proline, galactose, dopamine, Gamma-aminobutyric acid, muscimol, taurine) by the normal cat retina and retina injured by separation of neural retina and pigment epithelium. Changes in the uptake of these molecules with time after injury will be monitored to determine if we can find metabolic changes that parallel the morphological changes described by us and others. The distribution of cytoskeletal proteins (tubulin, microtubule-associated proteins, actin, neurofilaments, vimentin, glial fibrillary acidic protein) and surface saccharides will be studied in normal retina and isolated retinal cells by cytochemical studies using antibodies and lectins. Changes in the distribution of cytoskeletal proteins will also be studied after separation of neural retina and pigment epithelium and correlated with previously described morphological changes. We will use indirect fluorescence to detect lectin binding, indirect immunofluorescence, peroxidase-anti-peroxidase, protein A-gold, or immunogold techniques will be used to study antibody binding. Protein A-gold or immunogold techniques will be used to study antibody binding by electron microscopy. Monoclonal antibodies will be raised against cat retina and pigment epithelium and the localization of their specific antigens detemined immunocytochemically by light and electron microscopy. One of the most important long-term goals of this project is to use information obtained from these experiments to rescue retinal cells after retinal injury.