THE DEVELOPING AND ADULT VISUAL SYSTEM

发育中和成人的视觉系统

• 批准号: 3255587
• 负责人: STEVEN K FISHER
• 金额: $ 22.43万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-03-01 至 1989-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3255587
• 关键词: actins; autoradiography; carbohydrates; cats; cone cell; cytoskeleton; electron microscopy; eye injury; eye regeneration; fluorescence; glial fibrillary acidic protein; histochemistry /cytochemistry; hybridomas; immunochemistry; laboratory rabbit; light adaptations; microscopy; microtubule associated protein; microtubules; monoclonal antibody; neuroanatomy; neurofilament; neurogenesis; nonhistone nucleoprotein; retina; retina disorder; retinal pigment epithelium; retinaldehyde; silver impregnation; synapses; tubulin; vimentin; visual pathways

This project has several specific goals aimed at increasing our understanding of the vertebrate retina and its interdependence on the retinal pigment epithelium. These studies will contribute to our knowledge of retinal organization and to our understanding of the responses of the retina to injury. The effect of increased light intensity on cyclic cone disc shedding will be studied in ground squirrels. Synaptic organization and circuitry of the human retina will be studied in serial sections by electron microscopy, and the morphology of human retinal neurons studied by Golgi impregnation techniques. We will use light and electron microscope autoradiography to determine the patterns of uptake of various molecules (uridine, proline, galactose, dopamine, Gamma-aminobutyric acid, muscimol, taurine) by the normal cat retina and retina injured by separation of neural retina and pigment epithelium. Changes in the uptake of these molecules with time after injury will be monitored to determine if we can find metabolic changes that parallel the morphological changes described by us and others. The distribution of cytoskeletal proteins (tubulin, microtubule-associated proteins, actin, neurofilaments, vimentin, glial fibrillary acidic protein) and surface saccharides will be studied in normal retina and isolated retinal cells by cytochemical studies using antibodies and lectins. Changes in the distribution of cytoskeletal proteins will also be studied after separation of neural retina and pigment epithelium and correlated with previously described morphological changes. We will use indirect fluorescence to detect lectin binding, indirect immunofluorescence, peroxidase-anti-peroxidase, protein A-gold, or immunogold techniques will be used to study antibody binding. Protein A-gold or immunogold techniques will be used to study antibody binding by electron microscopy. Monoclonal antibodies will be raised against cat retina and pigment epithelium and the localization of their specific antigens detemined immunocytochemically by light and electron microscopy. One of the most important long-term goals of this project is to use information obtained from these experiments to rescue retinal cells after retinal injury.

该项目有几个具体目标，旨在提高我们的 了解脊椎动物的视网膜及其相互依赖的 视网膜色素上皮 这些研究将有助于我们了解 以及我们对视网膜组织反应的理解， 视网膜损伤。 提高光照强度对轮状花序的影响 将在地松鼠中研究圆盘脱落。 突触组织 和人类视网膜的电路将在连续切片中进行研究， 电子显微镜，和人类视网膜神经元的形态学研究， 高尔基体浸渍技术。 我们将使用光学和电子显微镜 放射自显影以确定各种分子的摄取模式 （尿苷，脯氨酸，半乳糖，多巴胺，γ-氨基丁酸，蝇蕈醇， 牛磺酸）的正常猫视网膜和视网膜分离损伤 神经视网膜和色素上皮。 这些变化的吸收 分子与受伤后的时间将被监测，以确定我们是否可以 发现代谢变化与以下描述的形态学变化平行： 我们和其他人 细胞骨架蛋白（微管蛋白， 微管相关蛋白，肌动蛋白，神经丝，波形蛋白，神经胶质 酸性蛋白质）和表面活性剂将在 正常视网膜和分离的视网膜细胞的细胞化学研究， 抗体和凝集素。 细胞骨架分布的变化 在分离神经视网膜和色素后，还将研究蛋白质 上皮，并与先前描述的形态学变化相关。 我们将使用间接荧光检测凝集素结合，间接 免疫荧光、过氧化物酶-抗过氧化物酶、蛋白A-金，或 免疫金技术将用于研究抗体结合。 蛋白 A-gold或免疫金技术将用于研究抗体结合， 电镜 将产生抗猫的单克隆抗体 视网膜和色素上皮及其特异性的定位 光镜和电镜免疫细胞化学检测抗原。 该项目最重要的长期目标之一是使用 从这些实验中获得的信息，以拯救视网膜细胞后， 视网膜损伤