GC Continuous Flow Isotope Ratio Mass Spectrometer

GC 连续流同位素比质谱仪

• 批准号: 6580510
• 负责人: Henri Brunengraber
• 金额: $ 25万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580510
• 关键词: biomedical equipment purchase; enzyme mechanism; fatty acid metabolism; gas chromatography mass spectrometry; gluconeogenesis; inborn metabolism disorder; ketone body; lipid biosynthesis; organic chemicals; protein metabolism

DESCRIPTION (provided by applicant): This application is for the acquisition of a continuous flow gas chromatograph combustion/ paralysis-isotope ratio mass spectrometer (GC-CF-IRMS) with modules for the on-line assay of low enrichments of compounds labeled with 13C, 15N, 18O, and 2H. The applicants are five established and two junior faculties of Case Western Reserve University and two colleagues from Youngstown State University (Ohio) and the University of Oklahoma, all with expertise in gas chromatography-mass spectrometry. The instrument will be used for basic science and clinical investigations on enzymatic reaction mechanisms, gluconeogenesis, lipogenesis, ketone body metabolism, protein metabolism, fatty acid oxidation, and inborn metabolic disorders. No such instrument is presently available at CWRU or in Greater Cleveland. The instrument will be set up in the Center for Metabolic Mass Spectrometry of the Department of Nutrition of CWRU. It will be made available to all scientists of the Cleveland area. The costs of operating and maintaining the instrument will be shared by the users. The availability of this instrument will considerably increase the scope of the basic science and clinical investigations conducted by the applicants.

描述（由申请人提供）：本申请用于获取连续流气相色谱燃烧/麻痹同位素比质谱仪（GC-CF-IRMS），该质谱仪具有用于13 C、15 N、18 O和2 H标记化合物低富集度在线分析的模块。申请人是凯斯西储大学的五名教师和两名初级教师，以及来自扬斯敦州立大学（俄亥俄州）和俄克拉荷马州大学的两名同事，他们都具有气相色谱-质谱分析方面的专业知识。该仪器将用于酶反应机制、脂肪生成、脂肪生成、酮体代谢、蛋白质代谢、脂肪酸氧化和先天性代谢紊乱的基础科学和临床研究。目前在CWRU或大克利夫兰没有这样的仪器。仪器将设在CWRU营养系代谢质谱中心。它将提供给克利夫兰地区的所有科学家。仪器的运行和维护费用将由用户分摊。该仪器的可用性将大大增加申请人进行的基础科学和临床研究的范围。