GC Continuous Flow Isotope Ratio Mass Spectrometer

描述（由申请人提供）：本申请是为了获得一台连续流气相色谱仪燃烧/麻痹同位素比质谱仪（GC-CF-IRMS），其模块用于在线分析标记为13C， 15N， 18O和2H的低富集化合物。申请者是凯斯西储大学的5名资深教员和2名初级教员，以及来自扬斯敦州立大学（俄亥俄州）和俄克拉何马大学的2名同事，他们都具有气相色谱-质谱分析方面的专业知识。该仪器将用于酶促反应机制、糖异生、脂肪生成、酮体代谢、蛋白质代谢、脂肪酸氧化和先天性代谢疾病的基础科学和临床研究。目前在CWRU或大克利夫兰没有这样的仪器。该仪器将设在中国农业大学营养系代谢质谱中心。它将提供给克利夫兰地区的所有科学家。操作和维护仪器的费用将由用户分担。该仪器的可用性将大大增加申请人进行的基础科学和临床研究的范围。

DESCRIPTION (provided by applicant): This application is for the acquisition of a continuous flow gas chromatograph combustion/ paralysis-isotope ratio mass spectrometer (GC-CF-IRMS) with modules for the on-line assay of low enrichments of compounds labeled with 13C, 15N, 18O, and 2H. The applicants are five established and two junior faculties of Case Western Reserve University and two colleagues from Youngstown State University (Ohio) and the University of Oklahoma, all with expertise in gas chromatography-mass spectrometry. The instrument will be used for basic science and clinical investigations on enzymatic reaction mechanisms, gluconeogenesis, lipogenesis, ketone body metabolism, protein metabolism, fatty acid oxidation, and inborn metabolic disorders. No such instrument is presently available at CWRU or in Greater Cleveland. The instrument will be set up in the Center for Metabolic Mass Spectrometry of the Department of Nutrition of CWRU. It will be made available to all scientists of the Cleveland area. The costs of operating and maintaining the instrument will be shared by the users. The availability of this instrument will considerably increase the scope of the basic science and clinical investigations conducted by the applicants.