GC Continuous Flow Isotope Ratio Mass Spectrometer

GC 连续流同位素比质谱仪

• 批准号: 6580510
• 负责人: Henri Brunengraber
• 金额: $ 25万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580510
• 关键词: biomedical equipment purchase; enzyme mechanism; fatty acid metabolism; gas chromatography mass spectrometry; gluconeogenesis; inborn metabolism disorder; ketone body; lipid biosynthesis; organic chemicals; protein metabolism

DESCRIPTION (provided by applicant): This application is for the acquisition of a continuous flow gas chromatograph combustion/ paralysis-isotope ratio mass spectrometer (GC-CF-IRMS) with modules for the on-line assay of low enrichments of compounds labeled with 13C, 15N, 18O, and 2H. The applicants are five established and two junior faculties of Case Western Reserve University and two colleagues from Youngstown State University (Ohio) and the University of Oklahoma, all with expertise in gas chromatography-mass spectrometry. The instrument will be used for basic science and clinical investigations on enzymatic reaction mechanisms, gluconeogenesis, lipogenesis, ketone body metabolism, protein metabolism, fatty acid oxidation, and inborn metabolic disorders. No such instrument is presently available at CWRU or in Greater Cleveland. The instrument will be set up in the Center for Metabolic Mass Spectrometry of the Department of Nutrition of CWRU. It will be made available to all scientists of the Cleveland area. The costs of operating and maintaining the instrument will be shared by the users. The availability of this instrument will considerably increase the scope of the basic science and clinical investigations conducted by the applicants.

描述（由申请人提供）：此申请是为了获取连续的流量气相色谱燃烧/瘫痪 - 同位素比率质谱仪（GC-CF-Irms），该模块用于在线测定，该测定在线测定低富集，标记为13C，15N，18O，18O和2H。申请人是五个成立的案件和两名凯斯西部储备大学的初级学院，纽斯敦州立大学（俄亥俄州）和俄克拉荷马大学的两位同事都拥有气相色谱质量光谱法专业知识。该仪器将用于基础科学和临床研究，讨论酶促机制，糖异生，脂肪生成，酮体代谢，蛋白质代谢，脂肪酸氧化和先天代谢疾病。目前在CWRU或大克利夫兰尚无此类乐器。该仪器将在CWRU营养系代谢质谱中心设置。它将提供给克利夫兰地区的所有科学家。操作和维护仪器的成本将由用户分担。该工具的可用性将大大增加申请人进行的基础科学和临床研究的范围。