Analytical Core

分析核心

• 批准号: 8379010
• 负责人: Henri Brunengraber
• 金额: $ 40.75万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8379010
• 关键词: Acyl Coenzyme A; Animal Experiments; Biochemical; Biological Markers; Body Fluids; Carbon; Citric Acid Cycle; Clinical; Complement; Data; Enzymes; Esters; Hydroxy Acids; Individual; Isotopes; Kinetics; Label; Link; Mass Spectrum Analysis; Measurement; Mentors; Metabolic; Metabolism; Mus; Organ; Pathway interactions; Pattern; Phenotype; Physiological Processes; Procedures; Regulation; Research Design; Scientist; Shipping; Ships; Techniques; Testing; Tissues; Training; Variant; acylcarnitine; design; in vivo; metabolic abnormality assessment; metabolomics; stable isotope; web page

ABSTRACT: Core C: Analytical & Metabolomic The Case MMPC specializes in the use of stable isotopes, mass spectrometry and metabolomics to investigate the regulation of pathways of intermediary metabolism in vivo and ex vivo. Emphasis is on quantitative measurements of inter-organ and intra-organ carbon fluxes. Our strategy links clinical-type studies (limited to body fluids) and pure biochemical studies (such as the kinetics of purified enzymes). The association of metabolomics and mass isotopomer analysis provides a wealth of information on the regulation of known pathways. It also allows to identify new pathways. Metabolomics developed initially as a non-targeted strategy aimed at gathering sufficient data on a (patho)physiological process (biomarkers), so that one could formulate hypotheses to be tested. A variant of this strategy is to target metabolomic studies to classes of compounds, the concentrations and labeling patterns of which can provide much new information: citric acid cycle intermediates, (hydroxy)acids, acyl-CoA esters, acylcarnitine esters. The Case MMPC occupies a specialized niche in the MMPC consortium, complementing the expertise of the other centers, but with a healthy degree of overlap with the Vanderbiit, Yale, Dallas and Cincinnati centers. The specific aims of the Analytical & Metabolomic Core are: 1. To help users in the design, implementation, analytical procedures and interpretation of metabolic studies conducted either, (i) entirely in the user's lab, (ii) entirely in the Case MMPC, or (iii) partly in the user's lab (animal experiments) and partly in the MMPC (analyses of tissues and body fluids shipped from the user's lab). 2. To set up, at users' request, tests and analytical procedures not currently listed on the web page of the Case MMPC. 3. To develop analytical techniques and isotopic strategies in anticipation of users' needs. 4. To train US and foreign scientists in the proper use of isotopes for metabolic studies. This training takes to form of (i) mentoring individual users, and (ii) contribution to an annual MMPC course.

摘要：核心C：分析与代谢 Case MMPC专门使用稳定同位素、质谱学和代谢组学来研究体内和体外中间代谢途径的调节。重点是器官间和器官内碳通量的定量测量。我们的策略将临床类型研究(仅限于体液)和纯生化研究(如纯化酶的动力学)联系起来。联谊会 代谢组学和质量同位异体分析为已知途径的调控提供了丰富的信息。它还允许识别新的途径。代谢组学最初是作为一种无针对性的策略发展起来的，目的是收集关于(病理)生理过程(生物标记物)的足够数据，以便人们可以制定待检验的假设。这一策略的一种变种是将代谢学研究瞄准一类化合物， 它们的浓度和标记模式可以提供许多新的信息：柠檬酸循环中间体、(羟基)酸、酰基辅酶A酯、酰肉碱酯。凯斯MMPC在MMPC财团中占据了一个专门的利基市场，与其他中心的专业知识相辅相成，但与范德比特、耶鲁、达拉斯和辛辛那提中心的重叠程度良好。 分析与代谢核心的具体目标是： 1.帮助用户设计、实施、分析程序和解释进行的代谢研究，(I)完全在用户实验室进行，(Ii)完全在MMPC情况下进行，或(Iii)部分在用户实验室(动物实验)和部分在MMPC(从用户实验室运送的组织和体液的分析)中进行。 2.应用户要求，设立目前未列在案例MMPC网页上的测试和分析程序。 3.根据用户的需要制定分析技术和同位素战略。 4.培训美国和外国科学家在代谢研究中正确使用同位素。这种培训的形式是(I)指导个人用户，以及(Ii)为年度MMPC课程做出贡献。