Analytical Core

摘要：核心C：分析和代谢组学 Case MMPC专门使用稳定同位素、质谱和代谢组学研究体内和体外中间代谢途径的调节。重点是对器官间和器官内碳通量的定量测量。我们的策略将临床类型研究（限于体液）和纯生化研究（如纯化酶的动力学）联系起来。联盟 代谢组学和质量同位素异构体分析提供了关于已知途径的调节的丰富信息。它还可以识别新的路径。代谢组学最初是作为一种非靶向策略发展起来的，旨在收集有关（病理）生理过程（生物标志物）的足够数据，以便人们可以制定待测试的假设。这种策略的一个变体是将代谢组学研究目标定在化合物的类别上， 其浓度和标记模式可以提供许多新的信息：柠檬酸循环中间体，（羟基）酸，酰基辅酶A酯，酰基肉毒碱酯。案例MMPC在MMPC联盟中占据了一个专门的利基市场，补充了其他中心的专业知识，但与范德比尔特、耶鲁、达拉斯和辛辛那提中心有着良好的重叠程度。 分析和代谢组学核心的具体目标是： 1.帮助用户设计、实施、分析程序和解释代谢研究，（i）完全在用户实验室中进行，（ii）完全在Case MMPC中进行，或（iii）部分在用户实验室（动物实验）部分在MMPC中进行（分析从用户实验室运送的组织和体液）。 2.应用户要求，设置当前未在Case MMPC网页上列出的检测和分析程序。 3.根据用户的需要发展分析技术和同位素战略。 4.培训美国和外国科学家正确使用同位素进行代谢研究。这种培训采取的形式是（一）指导个人用户，和（二）为年度MMPC课程提供帮助。

ABSTRACT: Core C: Analytical & Metabolomic The Case MMPC specializes in the use of stable isotopes, mass spectrometry and metabolomics to investigate the regulation of pathways of intermediary metabolism in vivo and ex vivo. Emphasis is on quantitative measurements of inter-organ and intra-organ carbon fluxes. Our strategy links clinical-type studies (limited to body fluids) and pure biochemical studies (such as the kinetics of purified enzymes). The association of metabolomics and mass isotopomer analysis provides a wealth of information on the regulation of known pathways. It also allows to identify new pathways. Metabolomics developed initially as a non-targeted strategy aimed at gathering sufficient data on a (patho)physiological process (biomarkers), so that one could formulate hypotheses to be tested. A variant of this strategy is to target metabolomic studies to classes of compounds, the concentrations and labeling patterns of which can provide much new information: citric acid cycle intermediates, (hydroxy)acids, acyl-CoA esters, acylcarnitine esters. The Case MMPC occupies a specialized niche in the MMPC consortium, complementing the expertise of the other centers, but with a healthy degree of overlap with the Vanderbiit, Yale, Dallas and Cincinnati centers. The specific aims of the Analytical & Metabolomic Core are: 1. To help users in the design, implementation, analytical procedures and interpretation of metabolic studies conducted either, (i) entirely in the user's lab, (ii) entirely in the Case MMPC, or (iii) partly in the user's lab (animal experiments) and partly in the MMPC (analyses of tissues and body fluids shipped from the user's lab). 2. To set up, at users' request, tests and analytical procedures not currently listed on the web page of the Case MMPC. 3. To develop analytical techniques and isotopic strategies in anticipation of users' needs. 4. To train US and foreign scientists in the proper use of isotopes for metabolic studies. This training takes to form of (i) mentoring individual users, and (ii) contribution to an annual MMPC course.