Confocal Microscope

描述（由申请人提供）：NIH研究人员在布朗的研究能力受到有限的共聚焦显微镜访问和技术的影响。在过去的几年里，研究人员不得不推迟实验，前往该地区以外的其他机构进行实验，最大限度地减少实验方案以适应现有的限制，并将项目的共聚焦成像外包出去。这一缺陷造成了美国国立卫生研究院研究人员的支持在这里的使用效率很低。显微镜的应用也扩大了许多调查人员和可用的，有限的仪器无法满足这些需求。我们认为，如果没有得到NCRR的支持，就没有其他办法获得这种仪器。被确定为主要用户的高级教师已经合作创建了此资金请求，以支持收购集成共聚焦显微镜系统。主要用户组由12个不同的实验室和86名研究人员组成。 建议的仪器系统包括以下内容：1。Zeiss LSM 510 Meta共聚焦显微镜，2.通过Zeiss Axiovert 200 MOT倒置显微镜成像，3.配备Narishige显微操作器和Eppendorf微量注射器，4.显微镜载物台温度控制系统。所要求的仪器将大大提高这里所述的广泛的跨学科研究人员群体的研究生产力，并大大提高该区域的生物成像能力。

DESCRIPTION (provided by applicant): The research capabilities of NIH investigators here at Brown are compromised by limited confocal microscope access and technology. Over the past several years, researchers have had to postpone experiments, travel to other institutions outside of the region to perform experiments, minimize the experimental protocol to accommodate the existing limitations, and farm out the confocal imaging of projects. This deficiency has created greatly inefficient use of NIH research support of investigators here. The applications of microcopy have also expanded for many investigators and the available, limited instrumentation is incapable of meeting these demands. We see no other way of acquiring such instrumentation without receiving this support from the NCRR. Twelve-faculty members, identified as Major Users, have collaborated to create this request for funds to support the acquisition of an integrated confocal microscope system. The Major User Group consists of twelve different laboratories with 86 researchers. The proposed instrument system contains the following: 1. Zeiss LSM 510 META confocal microscope, 2. Imaged through a Zeiss Axiovert 200 MOT inverted microscope, 3. Equipped with Narishige micromanipulators and an Eppendorf microinjector, 4. Microscope stage temperature control system. The requested instrument would significantly enhance the research productivity of the broad, interdisciplinary community of researchers described here, and greatly improve the biological image capabilities in the region.