Mechanisms of specification, quiescence, and regeneration of primordial germ cells

原始生殖细胞的规范、静止和再生机制

• 批准号: 10797823
• 负责人: GARY M WESSEL
• 金额: $ 11.29万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797823
• 关键词: 16 year old; Administrative Supplement; Adult; Animals; Biochemical; Biology; Biomedical Research; CRISPR/Cas technology; Cell Transplantation; Cells; Cellular biology; Characteristics; Chills; Chordata; Coupled; Development; Drops; Echinodermata; Embryo; Embryonic Development; Evaluation; Female; Genes; Genomics; Metabolic; Molecular; Morphology; Natural regeneration; Oligonucleotides; Organism; Parents; Process; Reagent; Research Support; Sea Urchins; Sexual Reproduction; Sister; Specific qualifier value; Starfish; Structure of primordial sex cell; System; Tissue Transplantation; Work; animal resource; aquarium; experimental study; genetic manipulation; in vivo; longitudinal analysis; mRNA sequencing; male; marine; optogenetics; serial imaging; small molecule

Project Summary We request support through this administrative supplement mechanism for a chilled marine aquarium unit to house our several echinoderm species, and our genetically manipulated adults derived from the experiments proposed in the parent application. Our 16-year-old main marine aquarium unit has failed, and our work has significantly expanded beyond its capacity and here we request support to replace it with a unit that will give us functionality and expanded capacity. The research supported by this request is focused on understanding the mechanisms of formation of primordial germ cells during embryogenesis, how they form during early development, and how they regenerate when the originals are removed. Our work leverages embryos from a sister group to chordates – the sea star and sea urchin. While not common organisms for biomedical research, these echinoderms have many strategic benefits for revealing unique perspectives in the biology of germline formation and regeneration. Millions of synchronous embryos from a single male/female cross allow biochemical and metabolic analysis of the germline, the resultant embryos have ideal transparency for in vivo longitudinal imaging, they develop rapidly, are easy to manipulate (single cell drop-mRNA-seq, optogenetics, cell and tissue transplantations) and they are well suited to complementary gene perturbation approaches (CRISPR/Cas9, morpholinoantisense oligonucleotides, MASO), and small molecule perturbations. The existing deep genomic and reagent resources for these animals, coupled with their tractable experimental characteristics, yields a unique system for understanding primordial germ cell biology with defined molecular and morphological endpoints, in live embryos with longitudinal analysis, distinct metrics of quantitation, and transgenerational evaluations.

项目总结

项目成果

Optimizing CRISPR/Cas9-based gene manipulation in echinoderms.

优化棘皮动物中基于 CRISPR/Cas9 的基因操作。

• DOI: 10.1016/j.ydbio.2022.07.008
• 发表时间: 2022
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Oulhen N;Pieplow C;Perillo M;Gregory P;Wessel, GM
• 通讯作者: Wessel, GM

Single-Cell Transcriptome and Pigment Biochemistry Analysis Reveals the Potential for the High Nutritional and Medicinal Value of Purple Sea Cucumbers.

• DOI: 10.3390/ijms241512213
• 发表时间: 2023-07-30
• 期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
• 影响因子: 5.6
• 作者: Xing, Lili;Wang, Lingyu;Liu, Shilin;Sun, Lina;Wessel, Gary M.;Yang, Hongsheng
• 通讯作者: Yang, Hongsheng

Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond.

• DOI: 10.1007/978-1-0716-0974-3_12
• 发表时间: 2021
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Perillo M;Paganos P;Spurrell M;Arnone MI;Wessel GM
• 通讯作者: Wessel GM

Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ.

• DOI: 10.1038/s41467-023-37947-2
• 发表时间: 2023-05-09
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Perillo, Margherita;Swartz, S. Zachary;Pieplow, Cosmo;Wessel, Gary M.
• 通讯作者: Wessel, Gary M.

A conserved node in the regulation of Vasa between an induced and an inherited program of primordial germ cell specification.

在诱导的原始生殖细胞规范和遗传程序之间对VASA调节中的保守节点。

• DOI: 10.1016/j.ydbio.2021.11.007
• 发表时间: 2022-03
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Perillo M;Swartz SZ;Wessel GM
• 通讯作者: Wessel GM