Macromolecular X-ray Structure Facility Instrumentation

描述(由申请人提供)： 将购买最先进的成像板面探测器，具有可变20级、低温冷却设备和共焦多层光学器件，以取代较旧的仪器。它将用于在配备铜靶的旋转阳极X射线发生器上收集单晶的X射线衍射数据。该装置将用于扩展和增强位于伊利诺伊大学芝加哥分校(UIC)分子生物学研究大楼(MBRB)的饱和多用户、大分子、单晶、X射线衍射设施的能力。该机构是UIC/RRC(研究资源中心)高分子结构设施(MSF)，位于西部医学院校园，目前支持七个主要研究小组(Gettins，Jeffery，Johnson，Lavie，MatSumura，Mesecar，Volz)，包括两个NIH计划拨款和一个NIH中心。大约80%的仪器时间将被NIH资助的研究人员使用，其余的时间将供UIC校园内的其他研究人员、芝加哥地区的研究人员和北卡罗来纳州的合作者使用。目前的研究设施是四年前建立的，用于增加药物化学、生药学、微生物学、生物化学和药物生物技术的课程。校园内外的新用户对该仪器的使用都由一个行政监督和咨询委员会管理，该委员会包括校园研究人员和RRC人员。目前运行的两个区域探测器和光学元件使用量很大，有些过时和老化，导致大量停机和维护增加。即将更换的西门子多线式区域探测器没有低温冷却系统，因此不适合低温储存或不稳定的样品。新的探测器和最先进的光学系统相结合，将允许对常规和困难样品进行更快、更准确的内部数据收集，包括长细胞轴、小晶体尺寸或较差的衍射样品，并为同步加速器时间准备提供更快、更彻底的晶体筛选。UIC/RRC MSF设施的使用增加反映了结构生物学作为芝加哥伊利诺伊斯大学研究和教学的主要组成部分的重要性日益增长。UIC的结构生物学研究的增长是由大学管理部门和结构生物学社区推动的，并反映在药物生物技术中心的建立和最近结构生物学中心的建立上。

DESCRIPTION (provided by applicant): A state-of-the-art imaging plate area detector with variable 20 stage, low-temperature cooling apparatus, and confocal multilayer optics will be purchased to replace older instrumentation. It will be used to collect x-ray diffraction data from single crystals on a rotating-anode x-ray generator equipped with a Cu target. The unit will be used to expand and enhance the capabilities of the saturated multi-user, macromolecular, single crystal, x-ray diffraction facility in the Molecular Biology Research Building (MBRB) at the University of Illinois at Chicago (UIC). This facility, the UIC/RRC (Research Resources Center) Macromolecular Structure Facility (MSF), is located on the west medical campus, and currently supports seven (Gettins, Jeffery, Johnson, Lavie, Matsumura, Mesecar, Volz) major research groups, including two NIH program grants and one NIH center. Approximately 80% of the instrument time will be utilized by NIH-funded researchers, and the remaining time will be available for other researchers on the UIC campus, in the Chicago area, and for a collaborator in North Carolina. The present research facility was established four years ago, and is used to augment courses in Medicinal Chemistry, Pharmacognosy, Microbiology, Biochemistry, and Pharmaceutical Biotechnology. Access to the instrumentation by new users both on and off campus has been governed by an administrative oversight and advisory committee including campus researchers and RRC personnel. The two area detectors and optics now in operation are heavily used, and are somewhat dated and aging, resulting in substantial downtime and increased maintenance. The Siemens multi-wire area detector, that will be replaced, has no low-temperature cooling system, and is therefore unsuitable for cryo-stored or unstable samples. The new detector and state-of-the-art optics system combination will allow for faster and more accurate in-house data collection on both routine and difficult samples including those with long cell axes, small crystal size, or poor diffraction, as well as provide for faster and more thorough screening of crystals in preparation for synchrotron time. The increased use of the UIC/RRC MSF facility reflects the growing importance of structural biology as a major component in research and teaching at the University of Illiniois at Chicago. The growth in structural biology research at UIC is being fostered by the University Administration and the structural biology community, and is reflected in the establishment of the Center for Pharmaceutical Biotechnology, and most recently the establishment of the Center for Structural Biology.