Macromolecular X-ray Structure Facility Instrumentation

高分子X射线结构设备仪器

• 批准号: 6578685
• 负责人: ANDREW D MESECAR
• 金额: $ 34.64万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6578685
• 关键词: X ray crystallography; biomedical equipment purchase; data collection methodology /evaluation; macromolecule; monitoring device; structural biology

DESCRIPTION (provided by applicant): A state-of-the-art imaging plate area detector with variable 20 stage, low-temperature cooling apparatus, and confocal multilayer optics will be purchased to replace older instrumentation. It will be used to collect x-ray diffraction data from single crystals on a rotating-anode x-ray generator equipped with a Cu target. The unit will be used to expand and enhance the capabilities of the saturated multi-user, macromolecular, single crystal, x-ray diffraction facility in the Molecular Biology Research Building (MBRB) at the University of Illinois at Chicago (UIC). This facility, the UIC/RRC (Research Resources Center) Macromolecular Structure Facility (MSF), is located on the west medical campus, and currently supports seven (Gettins, Jeffery, Johnson, Lavie, Matsumura, Mesecar, Volz) major research groups, including two NIH program grants and one NIH center. Approximately 80% of the instrument time will be utilized by NIH-funded researchers, and the remaining time will be available for other researchers on the UIC campus, in the Chicago area, and for a collaborator in North Carolina. The present research facility was established four years ago, and is used to augment courses in Medicinal Chemistry, Pharmacognosy, Microbiology, Biochemistry, and Pharmaceutical Biotechnology. Access to the instrumentation by new users both on and off campus has been governed by an administrative oversight and advisory committee including campus researchers and RRC personnel. The two area detectors and optics now in operation are heavily used, and are somewhat dated and aging, resulting in substantial downtime and increased maintenance. The Siemens multi-wire area detector, that will be replaced, has no low-temperature cooling system, and is therefore unsuitable for cryo-stored or unstable samples. The new detector and state-of-the-art optics system combination will allow for faster and more accurate in-house data collection on both routine and difficult samples including those with long cell axes, small crystal size, or poor diffraction, as well as provide for faster and more thorough screening of crystals in preparation for synchrotron time. The increased use of the UIC/RRC MSF facility reflects the growing importance of structural biology as a major component in research and teaching at the University of Illiniois at Chicago. The growth in structural biology research at UIC is being fostered by the University Administration and the structural biology community, and is reflected in the establishment of the Center for Pharmaceutical Biotechnology, and most recently the establishment of the Center for Structural Biology.

描述（由申请人提供）： 一个国家的最先进的成像板面积检测器与可变20阶段，低温冷却装置，共焦多层光学将购买，以取代旧的仪器。它将被用来收集X射线衍射数据从单晶上的阳极X射线发生器配备了一个铜目标。该装置将用于扩展和增强芝加哥伊利诺伊大学（UIC）分子生物学研究大楼（MBRB）的饱和多用户、大分子、单晶、X射线衍射设施的能力。这个设施，UIC/RRC（研究资源中心）大分子结构设施（MSF），位于西部医学园区，目前支持七个（Gettins，Jeffery，约翰逊，Lavie，Matsumura，Mesecar，Volz）主要研究小组，包括两个NIH计划赠款和一个NIH中心。大约80%的仪器时间将由NIH资助的研究人员使用，剩余的时间将提供给UIC校园，芝加哥地区的其他研究人员以及北卡罗来纳州的合作者。目前的研究设施是四年前建立的，用于增加药物化学，生药学，微生物学，生物化学和药物生物技术的课程。校园内外的新用户对仪器的访问由一个包括校园研究人员和RRC人员在内的行政监督和咨询委员会管理。现在运行的两个区域探测器和光学器件使用量很大，有些过时和老化，导致大量停机和维护增加。将被替换的西门子多线面积检测器没有低温冷却系统，因此不适合低温储存或不稳定的样品。新的探测器和最先进的光学系统组合将允许对常规和困难样品进行更快，更准确的内部数据收集，包括那些具有长细胞轴，小晶体尺寸或衍射差的样品，以及为准备同步加速器时间提供更快，更彻底的晶体筛选。UIC/RRC MSF设施的使用越来越多，反映了结构生物学作为芝加哥伊利诺伊大学研究和教学的主要组成部分的重要性日益增加。UIC结构生物学研究的增长正在由大学管理和结构生物学社区促进，并反映在药物生物技术中心的建立，以及最近的结构生物学中心的建立。

项目成果

Evaluating the 3C-like protease activity of SARS-Coronavirus: recommendations for standardized assays for drug discovery.

• DOI: 10.1016/j.virusres.2007.02.015
• 发表时间: 2008-04
• 期刊: VIRUS RESEARCH
• 影响因子: 5
• 作者: Grum-Tokars, Valerie;Ratia, Kiira;Begaye, Adrian;Baker, Susan C.;Mesecar, Andrew D.
• 通讯作者: Mesecar, Andrew D.

Structural basis for catalysis of a tetrameric class IIa fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis.

• DOI: 10.1016/j.jmb.2009.01.003
• 发表时间: 2009-03-06
• 期刊: JOURNAL OF MOLECULAR BIOLOGY
• 影响因子: 5.6
• 作者: Pegan, Scott D.;Rukseree, Kamolchanok;Franzblau, Scott G.;Mesecar, Andrew D.
• 通讯作者: Mesecar, Andrew D.

Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.

• DOI: 10.1016/j.abb.2009.06.005
• 发表时间: 2009-08-15
• 期刊: Archives of biochemistry and biophysics
• 影响因子: 3.9
• 作者: Hawwa R;Aikens J;Turner RJ;Santarsiero BD;Mesecar AD
• 通讯作者: Mesecar AD