权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

LEICA TCS SP2 ADBS CONFOCAL MICROSCOPE

LEICA TCS SP2 ADBS 共焦显微镜

基本信息

批准号：
6581134
负责人：
JAMES A. BOROWIEC
金额：
$ 36.05万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-04-01 至 2004-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6581134
关键词：
biochemistry biomedical equipment purchase confocal scanning microscopy microbiology

项目摘要

DESCRIPTION (provided by applicant): The application requests support for a Leica TCS SP2 AOBS Spectral Confocal Microscope. The recently introduced spectrally programmable Leica AOBS technology allows a filter-free operation, which increases the signal strength allowing reduced photobleaching and longer cell lifetimes. The microscope will be a shared facility used by members of the Departments of Microbiology (4 major and 1 minor users) and Biochemistry (3 major and 2 minor users) at New York University School of Medicine (NYU SOM). Between them, the users have 18 NIH grants. The proposed studies of these investigators primarily characterize the components of signaling pathways that regulate cell growth in normal and virally-infected or stressed cells. These projects involve the determination of sub-cellular localization of proteins in living and fixed cells, and analysis in living cells of nucleocytoplasmic shuttling using FRAP and interactions of multiple protein factors using FRET. The projects of the major users include studies of: i) transcriptional control of lytic and latent gene expression in herpesviruses (Wilson), ii) modulation of mRNA translation by Us11 in herpesvirus-infected cells (Mohr), iii) maintenance of a non-replicating Hepatitis B virus DNA episome in the infected cell nucleus (Schneider), iv) characterization of a novel mechanism for regulating p53 stability through a nucleolin-p53 interaction (Borowiec), v) the mechanism of retinal degeneration caused by defects in RP2, a causative factor in retinitis pigmentosa (Cowan), vi) localization of glucocorticoid receptor phosphorylation isoforms (Garabedian), and vii) trafficking of K+ channel proteins of the Kv4 subfamily and the dynamics of their interactions with associated proteins (Rudy). Significant progress towards these studies is currently prevented because confocal facilities at NYU SOM are heavily over-subscribed and access is severely limited. The success of these NIH-supported projects therefore requires full-time access to a high-resolution confocal microscope. As additional benefits, the instrument will enhance the training of students and postdoctoral fellows in the two departments, and provide increased synergy between user groups that will generate new research directions. The Department chairs and user groups have committed sufficient funds for instrument maintenance and training.

描述（由申请人提供）：该应用程序请求支持Leica TCS SP2 AOBS光谱共聚焦显微镜。最近推出的光谱可编程Leica AOBS技术允许无滤波器操作，这增加了信号强度，从而减少了光漂白并延长了电池寿命。该显微镜将是纽约大学医学院（NYU SOM）微生物学系（4名主要用户和1名次要用户）和生物化学系（3名主要用户和2名次要用户）成员使用的共享设施。在他们之间，用户有18个NIH赠款。这些研究人员的拟议研究主要表征调节正常和病毒感染或应激细胞中细胞生长的信号通路的组分。这些项目包括确定活细胞和固定细胞中蛋白质的亚细胞定位，以及使用FRAP分析活细胞中的核质穿梭和使用FRET分析多种蛋白质因子的相互作用。主要用户的项目包括以下研究：i）疱疹病毒中裂解和潜伏基因表达的转录控制（Wilson），ii）疱疹病毒感染细胞中Us 11对mRNA翻译的调节（Mohr），iii）在感染细胞核中维持非复制型B肝炎病毒DNA附加体（Schneider），iv）通过核仁-p53相互作用调节p53稳定性的新机制的表征（Borowiec），v）视网膜色素变性的致病因子RP 2缺陷引起的视网膜变性的机制（科万），vi）糖皮质激素受体磷酸化亚型的定位（Garabedian），和vii）Kv 4亚家族的K+通道蛋白的运输及其与相关蛋白相互作用的动力学（Rudy）。这些研究的重大进展目前被阻止，因为纽约大学SOM的共聚焦设施严重超额认购，访问受到严重限制。因此，这些NIH支持的项目的成功需要全职使用高分辨率共聚焦显微镜。作为额外的好处，该工具将加强两个部门的学生和博士后研究员的培训，并提供更多的用户群体之间的协同作用，将产生新的研究方向。该部主席和用户团体已承诺为仪器维护和培训提供足够的资金。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Quantitative analysis of BDNF/TrkB protein and mRNA in cortical and striatal neurons using ýý-tubulin as a normalization factor.

使用 α-微管蛋白作为标准化因子，对皮质和纹状体神经元中的 BDNF/TrkB 蛋白和 mRNA 进行定量分析。

DOI：
10.1002/cyto.a.22073
发表时间：
2012
期刊：
Cytometry. Part A : the journal of the International Society for Analytical Cytology
影响因子：
0
作者：
Ma,Bin;Savas,JeffreyN;Chao,MosesV;Tanese,Naoko
通讯作者：
Tanese,Naoko

The latency-associated nuclear antigen interacts with MeCP2 and nucleosomes through separate domains.

潜伏期相关核抗原通过不同的结构域与 MeCP2 和核小体相互作用。