Regulation of RPA Activity in DNA Repair

DNA 修复中 RPA 活性的调节

• 批准号: 8668405
• 负责人: JAMES A. BOROWIEC
• 金额: $ 32.42万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8668405
• 关键词: BRCA2 gene; Binding; Cell Cycle; Cell Cycle Progression; Charge; Complex; Cultured Cells; Cyclin A; Cyclins; DNA; DNA Damage; DNA Repair; DNA Repair Pathway; DNA Replication Damage; DNA biosynthesis; DNA lesion; DNA replication fork; Data; Development; Event; Funding; Funding Opportunities; Genotoxic Stress; Goals; Human; In Vitro; Laboratories; Medical; Mitosis; Mitotic; Modification; Molecular; Mutation; National Institute of General Medical Sciences; New York City; Pathway interactions; Phosphorylation; Phosphorylation Site; Phosphotransferases; Physiological; Positioning Attribute; Proteins; Reaction; Recruitment Activity; Regulation; Reporting; Research; Role; SS DNA BP; Science; Site; Solutions; Stress; Structure; System; Testing; Tumor Suppressor Proteins; United States National Institutes of Health; Variant; Work; biological adaptation to stress; cost; design; experience; helicase; homologous recombination; in vivo; insight; medical schools; mutant; operation; recombinational repair; replication factor A; research study; response; synthetic construct

Superstorm Sandy hit New York City on October 29, 2012. The resulting storm surge led to the closure of the Medical Science Building (MSB) at the NYU School of Medicine (SoM), in which my laboratory was located. The damage to MSB had a major impact on our research work performed in the months prior to the storm, as well as on laboratory operations during the subsequent months, with all of this work funded by R01 GM083185 (including an NIGMS Supplement for Collaborative Science). Because of this significant disruption of a full year of NIH funded activities, we request a one-year cost extension using funding opportunity RFA-OD-13-199.

2012年10月29日，超级风暴桑迪袭击了纽约市。由此产生的 风暴潮导致纽约大学医学院医学大楼(MSB)关闭 我的实验室就在那里。对MSB的破坏有一个重大的 对我们在风暴前几个月进行的研究工作以及对 随后几个月的实验室运行，所有这些工作都由R01提供资金 GM083185(包括NIGMS协作科学附录)。正因为如此 NIH资助的一整年活动严重中断，我们要求一年的费用 利用筹资机会延长RFA-OD-13-199。

项目成果

Specific domains of nucleolin interact with Hdm2 and antagonize Hdm2-mediated p53 ubiquitination.

• DOI: 10.1111/j.1742-4658.2011.08430.x
• 发表时间: 2012-02
• 期刊: The FEBS journal
• 作者: Bhatt P;d'Avout C;Kane NS;Borowiec JA;Saxena A
• 通讯作者: Saxena A

A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination.

• DOI: 10.1038/nsmb.1769
• 发表时间: 2010-03
• 期刊: Nature structural & molecular biology
• 影响因子: 16.8