Fluorescence Activated Cell Sorter

描述(由申请者提供)：本建议书要求为Lombardi癌症中心(LCC)流式细胞术/细胞分选共享资源购买一台FACSVantage SE流式细胞仪。该仪器旨在替换和升级1988年购买的现有和过时的FACStar Plus。该资源维护着校园内唯一可供研究人员使用的细胞仪，唯一具有可调谐激光和可更换发射滤光片的仪器，以及乔治敦大学(GU)唯一的荧光激活细胞分类器。目前的细胞仪FACStar Plus自1992年以来一直停产，获得维修部件变得越来越困难。拟议中的新细胞仪将允许进行NIH资助的项目所需的更复杂的多色分析，同时大幅提高细胞分选能力。TurboSort功能将允许调查人员对大量细胞进行分类，或分离出足够数量的罕见事件群体，但由于时间限制，现在无法对其进行分类。QuadraSort功能将在对多个种群进行排序时非常有用，特别是从细胞数量有限的样本中进行排序。另一项功能将允许对非矩形群体进行排序。在23个主要用户中，目前有8个项目需要现有仪器所不具备的能力。其他项目将通过这些功能得到增强。超过40个PI将大大受益于可靠的最先进的细胞仪的可用性。来自11个部门的调查人员大量使用了这一资源。2001年，43名研究人员利用该设施进行了750多项实验。目前，有29名调查人员使用该设施进行同行评审的NIH资助项目，还有5名调查人员使用其他资金来源的同行评审资助项目。该资源成立于1988年，由癌症中心资金、NCI CCSG赠款支持和用户费用共同支持。该资源由Karen Creswell博士领导，他在流式细胞仪方面有12年的经验，他将担任主要的仪器操作员。咨询委员会监督准入、费用、政策，并为未来的增长提出建议。LCC致力于支持这一资源，以确保持续的维护、优质的服务和广泛的GU社区访问。

DESCRIPTION (provided by applicant): This proposal requests the funds to purchase a FACSVantage SE flow cytometer for the Lombardi Cancer Center (LCC) Flow Cytometry/Cell Sorting Shared Resource. This instrument is intended as a replacement and upgrade for an existing and outdated FACStar Plus purchased in 1988. The Resource maintains the only cytometers accessible to researchers on the campus, the only instrument with tunable lasers and exchangeable emission filters as well as the only fluorescence activated cell sorters at Georgetown University (GU). The current cytometer, the FACStar Plus, has been out of production since 1992 and it is becoming more difficult to obtain repair parts. The proposed new cytometer will allow more complex multicolor analyses required for ongoing NIH funded projects along with great improvements in cell sorting capabilities. The TurboSort feature will allow investigators to sort out large numbers of cells or to isolate sufficient quantities of rare event populations, which are now not feasible to sort due to time constraints. The QuadraSort capability will be valuable in sorting multiple populations, particularly from samples with limited cell numbers. An additional feature will allow for the sorting of nonrectangular populations. Of the 23 major users, 8 currently have projects which require capabilities not available on the present instrumentation. Other projects would be enhanced by these features. More than 40 PIs would greatly benefit from the availability of a reliable state-of-the-art cytometer. The Resource is heavily utilized by investigators from 11 departments. In 2001, 43 investigators utilized the facility for more than 750 experiments. Currently, there are more than 29 investigators with peer-reviewed NIH funded projects utilizing the facility along with 5 investigators with peer reviewed funded projects from other funding sources. The Resource, established in 1988, is supported by a combination of Cancer Center funding, NCI CCSG grant support, and user fees. The Resource is headed by Dr. Karen Creswell, who has 12 years of experience in flow cytometry, and who will serve as the primary instrument operator. An advisory committee oversees access, fees, policies, and makes recommendations for future growth. The LCC is committed to support this Resource to ensure continued upkeep, excellent service and wide access to the GU community.