Fluorescence Activated Cell Sorter

描述（由申请人提供）：本申请申请为Lombardi Cancer Center （LCC）流式细胞术/细胞分选共享资源购买一台facvantage SE流式细胞仪的资金。该仪器旨在替代和升级1988年购买的现有和过时的FACStar Plus。该资源维护着校园内唯一可供研究人员使用的细胞仪，乔治城大学（GU）唯一具有可调谐激光器和可交换发射过滤器的仪器以及唯一的荧光激活细胞分选器。目前的细胞仪FACStar Plus自1992年以来已经停产，并且越来越难以获得修复部件。拟议的新细胞仪将允许更复杂的多色分析需要正在进行的NIH资助的项目随着细胞分选能力的巨大改进。TurboSort功能将允许研究人员对大量细胞进行分类，或分离出足够数量的罕见事件种群，由于时间限制，目前无法进行分类。QuadraSort的功能将是有价值的分选多个群体，特别是从有限的细胞数量的样本。一个附加功能将允许对非矩形种群进行排序。在23个主要用户中，目前有8个项目需要现有仪器无法提供的功能。其他项目将通过这些特性得到加强。40多个pi将大大受益于可靠的最先进的细胞仪的可用性。该资源由11个部门的调查人员大量使用。2001年，43名研究人员利用该设施进行了750多次实验。目前，有超过29名研究人员与同行评审的NIH资助项目利用该设施，还有5名研究人员与同行评审的其他资金来源的资助项目利用该设施。该资源成立于1988年，由癌症中心资助、NCI CCSG拨款支持和用户费用共同支持。该资源由拥有12年流式细胞术经验的Karen Creswell博士领导，他将担任主要的仪器操作员。咨询委员会负责监督准入、收费、政策，并为未来的增长提出建议。LCC致力于支持此资源，以确保持续维护，提供优质服务并广泛访问GU社区。

DESCRIPTION (provided by applicant): This proposal requests the funds to purchase a FACSVantage SE flow cytometer for the Lombardi Cancer Center (LCC) Flow Cytometry/Cell Sorting Shared Resource. This instrument is intended as a replacement and upgrade for an existing and outdated FACStar Plus purchased in 1988. The Resource maintains the only cytometers accessible to researchers on the campus, the only instrument with tunable lasers and exchangeable emission filters as well as the only fluorescence activated cell sorters at Georgetown University (GU). The current cytometer, the FACStar Plus, has been out of production since 1992 and it is becoming more difficult to obtain repair parts. The proposed new cytometer will allow more complex multicolor analyses required for ongoing NIH funded projects along with great improvements in cell sorting capabilities. The TurboSort feature will allow investigators to sort out large numbers of cells or to isolate sufficient quantities of rare event populations, which are now not feasible to sort due to time constraints. The QuadraSort capability will be valuable in sorting multiple populations, particularly from samples with limited cell numbers. An additional feature will allow for the sorting of nonrectangular populations. Of the 23 major users, 8 currently have projects which require capabilities not available on the present instrumentation. Other projects would be enhanced by these features. More than 40 PIs would greatly benefit from the availability of a reliable state-of-the-art cytometer. The Resource is heavily utilized by investigators from 11 departments. In 2001, 43 investigators utilized the facility for more than 750 experiments. Currently, there are more than 29 investigators with peer-reviewed NIH funded projects utilizing the facility along with 5 investigators with peer reviewed funded projects from other funding sources. The Resource, established in 1988, is supported by a combination of Cancer Center funding, NCI CCSG grant support, and user fees. The Resource is headed by Dr. Karen Creswell, who has 12 years of experience in flow cytometry, and who will serve as the primary instrument operator. An advisory committee oversees access, fees, policies, and makes recommendations for future growth. The LCC is committed to support this Resource to ensure continued upkeep, excellent service and wide access to the GU community.