LSR-Fortessa upgrade for Shared Resource

描述(由申请人提供)：本建议书申请资金为伦巴迪综合癌症中心(LCCC)流式细胞术/细胞分选共享资源购买Becton Dickinson LSR-Fortessa流式细胞仪。该仪器将增强该资源的能力和容量，该资源是乔治敦大学唯一的流式细胞仪。拟议的新细胞仪将允许进行更复杂的多色(多达13个荧光参数)分析，这是该大学正在进行的NIH资助的项目所需的。拟议的仪器将允许共享资源通过额外的多激光分析仪为乔治敦大学医学中心提供不间断的分类服务。详细介绍了来自共享资源的10个主要用户的研究项目。LCCC流式细胞术/细胞分选共享资源维护着乔治敦大学唯一的荧光激活细胞分选仪(FACS)。来自医学院和本科生校园各部门的调查人员大量利用了这一资源。2011年，46名研究人员利用该设施进行了1200多项实验。目前，有超过35名调查人员利用该设施开展了经同行审查的资助项目。共享资源成立于1988年，由癌症中心资金、NCI CCSG赠款支持和用户费用共同支持。一个咨询委员会监督费用、政策，并为未来的增长提出建议。

DESCRIPTION (provided by applicant): This proposal requests the funds to purchase a Becton Dickinson LSR-Fortessa flow cytometer for the Lombardi Comprehensive Cancer Center (LCCC) Flow Cytometry/Cell Sorting Shared Resource. This instrument will enhance the capabilities and capacity of the resource, which houses the only flow cytometers at Georgetown University. The proposed new cytometer will allow more complex multicolor (up to thirteen fluorescence parameters) analysis required for ongoing NIH funded projects in the University. The proposed instrument will allow the Shared Resource to provide uninterrupted sorting service to the Georgetown University Medical Center by the additional of this multilaser analyzer. Research projects from 10 major users of the Shared Resource are detailed. The LCCC Flow Cytometry/Cell Sorting Shared Resource maintains the only fluorescence activated cell sorter (FACS) at Georgetown University. This resource is heavily utilized by investigators from departments in the medical school and undergraduate campus. In 2011, 46 investigators utilized the facility for more than 1200 experiments. Currently, there are more than 35 investigators with peer- reviewed funded projects utilizing the facility. The Shared Resource, established in 1988, is supported by a combination of Cancer Center funding, NCI CCSG grant support, and user fees. An advisory committee oversees fees, policies, and makes recommendations for future growth.