Shared Resource - Flow Cytometry & Cell Sorting Shared Resource

共享资源 - 流式细胞仪

• 批准号: 10400646
• 负责人: KAREN A CRESWELL
• 金额: $ 4.34万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-15 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10400646
• 关键词: Academic Medical Centers; Aerosols; Apoptotic; Argon; Biological Assay; Cancer Center; Cancer Center Support Grant; Cell Cycle; Cell Separation; Cell physiology; Cell surface; Cells; Cellular Assay; Cloning; Collaborations; Color; Complex; Comprehensive Cancer Center; Computer software; Conflict (Psychology); Containment; Data Analyses; Deposition; Doctor of Philosophy; Ensure; Flow Cytometry; Flow Cytometry Shared Resource; Fluorescence; Fluorescence-Activated Cell Sorting; Funding; Future; Goals; Grant; Human Resources; Immune; Immunophenotyping; Immunotherapy; Informatics; Investigational Therapies; Ions; Label; Lasers; Liquid substance; Methods; Modeling; Mus; New Jersey; Oxidative Stress; Peer Review; Phenotype; Population; Research; Research Personnel; Research Project Grants; Research Support; Resource Sharing; Schedule; Services; Site Visit; Software Tools; Sorting - Cell Movement; Speed; Stains; Strategic Planning; System; Technology; Training; Transfection; Tumor-Infiltrating Lymphocytes; United States National Institutes of Health; Universities; Viola; Virginia; anticancer research; base; detector; enhanced green fluorescent protein; experimental study; human tissue; improved; innovation; instrument; instrumentation; malignant breast neoplasm; member; molecular oncology; novel strategies; oncology program; precision medicine; programs; solid state; stem cell biomarkers; stem cell population; tool

ABSTRACT The Flow Cytometry & Cell Sorting Shared Resource (FCSR) was established in 1988 to provide flow cytometry support to researchers of the Georgetown Lombardi Comprehensive Cancer Center (LCCC). The Cancer Center Support Grant (CCSG) has supported the FCSR since 1990. Karen Creswell, PhD, who has been with the FCSR since 1991, is director. The purpose of the FCSR is to apply a wide range of flow cytometric and fluorescence-activated cell sorting solutions to problems in cancer research. The FCSR provides comprehensive support to facilitate utilization of this technology by a variety of investigators. Specifically, the FCSR 1) maintains three flow cytometers/cell sorters, which are the only instruments with cell sorting capability at Georgetown University (Georgetown); 2) provides trained operators to ensure efficient and correct use of this complex instrumentation and to assist with data interpretation; 3) provides personnel to assist with applying a variety of fluorescence-labeling methods; and 4) provides expertise for the innovative application of this instrumentation to fully exploit its capabilities for cancer research. Since the last site visit, the FCSR has seen an increase in use and an expansion of projects with more complex phenotyping experiments. In fiscal year (FY) 2017, 20 peer review-funded researchers from three LCCC programs used FCSR services as important tools in their research. The FCSR provides value to research projects by separating cells based on transfection markers, such as enhanced green fluorescent protein, stem cell markers and other cell surface markers. Functional assays of the cell cycle, apoptotic status and oxidative stress are performed in addition to phenotyping. Immunophenotyping of mouse and human tissues and tumor- infiltrating lymphocytes (TILs) has increased due to the 14-color capabilities of the Fortessa. Through the Mid- Atlantic Shared Resource Consortium (MASRC), LCCC investigators have access to state-of-the-art CyTOF analyses and informatics solutions. These assays allow researchers to quickly and effectively assess the phenotype and function of cell populations in their research. Future plans for the FCSR include expanded support of immunotherapy research in collaboration with investigators at the John Theurer Cancer Center (JTCC) on the New Jersey campus of the LCCC Consortium (LCCC-NJ).

摘要 流式细胞术和细胞分选共享资源（FCSR）成立于1988年，提供流式细胞术 乔治城隆巴迪综合癌症中心（Georgetown Lombardi Comprehensive Cancer Center，LCCC）癌症中心 自1990年以来，支助赠款一直在支助《儿童权利公约》。凯伦·克雷斯韦尔博士， 自1991年起担任社研基金会主任。FCSR的目的是应用广泛的流式细胞术， 荧光激活细胞分选解决癌症研究中的问题。FCSR提供全面的 提供支助，以便利各种调查人员利用这一技术。具体而言，《财务报告准则》1）认为， 三台流式细胞仪/细胞分选仪，这是乔治敦唯一具有细胞分选能力的仪器 大学（乔治敦）; 2）提供训练有素的操作员，以确保有效和正确地使用这一复杂的 仪器和协助数据解释; 3）提供人员，以协助应用各种 荧光标记方法;和4）为这种仪器的创新应用提供专业知识， 充分发挥其在癌症研究方面的能力。 自上一次实地考察以来，FCSR的使用量有所增加，项目规模也有所扩大， 表型分析实验在2017财年，来自三个LCCC的20名同行评审资助的研究人员 项目将FCSR服务作为其研究的重要工具。FCSR为研究提供了价值 通过基于转染标记物分离细胞的项目，例如增强型绿色荧光蛋白、干细胞和细胞因子。 细胞标记和其它细胞表面标记。细胞周期、凋亡状态和氧化应激的功能测定 除了表型分析之外，还进行应激。小鼠和人类组织及肿瘤的免疫表型分析- 由于Fortessa的14色能力，浸润淋巴细胞（TIL）增加。通过中- 大西洋共享资源联盟（MASRC），LCCC研究人员可以使用最先进的CyTOF 分析和信息解决方案。这些测定使研究人员能够快速有效地评估 表型和功能的细胞群体在他们的研究。该基金的未来计划包括扩大支持 与约翰·特雷尔癌症中心（JTCC）的研究人员合作， LCCC联盟（LCCC-NJ）的新泽西校区。