High-Throughput Robotic Liquid Handling System

描述(由申请人提供)：NCRR共享仪器拨款申请的总体目标是增加机构共享DNA测序核心(DNAcore)的吞吐量，以增加机构范围内最先进的测序、基因分型和杂合性丢失(LOH)分析的能力，以促进和加强所有NIH资助的西奈山医学院研究人员的研究工作。具体要求是一个高通量机器人液体处理系统，用于自动准备样品以进行测序和基因分型。所要求的Velocity 11 Vprep TM系统是经过验证的高质量机器人工作站，具有极快的周期时间和极佳的灵活性，可用于多种应用，可支持130多名高生产率的西奈山调查人员的高通量需求。这一应用因其首席研究员在过去7年为人类遗传学系提供高质量DNA测序、基因分型和寡核苷酸合成方面的经验而得到加强，最初使用ABI 377XL自动DNA测序仪和ABI 394DNA合成仪，最近使用ABI 377XL、ABI 3100毛细管阵列测序仪、ABI 3700毛细阵列测序仪、Transgenology WAVE TM片段分析仪和Sun SPARC系统服务器在我们的机构以太网主干上为整个西奈山园区提供高质量的DNA测序、基因分型和寡核苷酸合成。有了这笔赠款，这一功能良好的核心将得到升级和增强，以满足我们列出的主要用户持有的129项国家卫生研究院赠款的快速扩展要求。DNAcore将为所有测序、基因分型和杂合性缺失项目提供内部专业知识和咨询服务。将通过DNAcore的机构咨询委员会促进对所要求的自动化液体处理服务的公平、成本效益和反应能力的利用。他们还将确定和推荐新的战略、技术、仪器和资源，以进一步促进和促进尖端研究。因此，DNAcore将满足研究人员对现场测序的强烈需求，以加快基因发现、基因结构分析、突变表征、载体构建分析等。总而言之，所需设备将与现有资源和我们院长的主要基因组学/生物信息学倡议协同配合，显著提高研究生产率。我们的专家和经验丰富的DNAcore董事和员工将有效、快速地将所需的高通量机器人系统集成到我们已建立的中央核心设施中，以显著提高其能力。

DESCRIPTION (provided by applicant): The overall objective of this NCRR Shared Instrument Grant application is to increase the throughput of the Institutional Shared DNA Sequencing Core (DNAcore) to provide increased capacity for Institution-wide, state-of-the-art sequencing, genotyping, and loss of heterozygosity (LOH) analyses to facilitate and enhance the research efforts of all NIH-funded Investigators at the Mount Sinai School of Medicine. The specific request is for a High-Throughput Robotic liquid handling system for automated sample preparation for sequencing and genotyping. The requested Velocity 11 Vprep TM system is a proven, high-quality robotic workstation with extremely rapid cycle times and excellent flexibility for use in multiple applications that can support the high-throughput needs of over 130 highly productive, Mount Sinai investigators. This application is strengthened by the previous 7 years of experience of its Principal Investigator in providing high-quality DNA sequencing, genotyping and oligonucleotide synthesis initially for the Faculty of the Department of Human Genetics using an ABI 377XL Automated DNA Sequencer and ABI 394 DNA synthesizer, and more recently, for the entire Mount Sinai campus using the ABI 377XL, ABI 3100 Capillary Array Sequencer, ABI 3700 Capillary Array Sequencer, Transgenomics WAVE TM fragment analyzer and Sun SPARC system server on our Institutional Ethernet backbone. With this grant, this well-functioning Core will be upgraded and enhanced to meet the rapidly expanding requirements of 129 NIH grants held by our 89 listed major users. The DNAcore will provide in-house expertise and consultation services for all sequencing, genotyping, and LOH projects. Equitable, cost-effective and responsive utilization of the requested automated liquid-handling services will be promoted through the DNAcore's Institutional Advisory Committee. They will also identify and recommend novel strategies, technologies, instrumentation, and resources to further facilitate and foster cutting-edge research. Thus, the DNAcore will meet intense needs of investigators for on-site sequencing to speed gene discovery, gene structure analysis, mutation characterization, analysis of vector constructs, etc. In summary, the requested equipment will fit synergistically with existing resources and our Dean's major genomics/bioinformatics initiatives to markedly enhance research productivity. Our expert and experienced DNAcore Directors and staff will effectively and rapidly integrate the requested high-throughput robotics system into our established central core facility to significantly increase its capabilities.