ABI 3730XL DNA ANALYZER: GENOMICS

ABI 3730XL DNA 分析仪：基因组学

• 批准号: 7335253
• 负责人: DAVID Franklin BISHOP
• 金额: $ 5.38万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-15 至 2007-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335253
• 关键词: DNA; gene mutation; genes; genotype; nucleic acid sequence

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The overall objective of this NCRR Shared Instrument Grant application is to upgrade and update a heavily-used Institutional Shared DNA Sequencing and Genotyping Core (DNAcore) with an ABI 3730xl DNA Analyzer to 1) lower costs of DNA sequencing and genotyping, 2) increase sequencing read-length and quality, and to 3) provide the new high-throughput ABI SNPlex method for SNP analysis. The requested ABI 3730xl DNA Analyzer is the current state-of-the-art capillary array analyzer that will fulfill all these goals and provide the economy and flexibility needed to satisfy the multitude of user projects requiring our shared resource facility. Of great importance to our investigators working on gene association projects, it will support low to high throughput genotyping utilizing a wide variety of approaches including STR-based linkage, single SNP analyses, allele quantitation by SNaPshot assays, and high throughput genotyping with 48-plex SNPlex assays. This upgrade to our busy shared sequencing, genotyping and oligonucleotide synthesis core facility at Mount Sinai will replace the ageing ABI 3700 platform that is too expensive to run due to its much greater use of reagents, its downtime due to hardware failures, its inability to support the SNPlex assay and its reduced signal sensitivity and uniformity relative to the new ABI 3730xl. This application is strengthened by the previous 10 years of experience of its Principal Investigator in providing high-quality DNA sequencing, genotyping, mutation detection and oligonucleotide synthesis for rapidly increasing numbers of NIH and other investigators at Mount Sinai. The DNAcore provides in-house expertise and consultation services for all sequencing, genotyping, positional cloning, mutation detection, and LOH projects. Equitable, cost-effective and responsive utilization of the requested instrumentation will be promoted through the DNAcore's Local Advisory Committee. They will also identify and recommend novel strategies, technologies, instrumentation, and resources to further facilitate and foster cutting-edge research. The Dean for Research will support service contracts and provide $100K towards the purchase of the requested ABI 3730xl, which with the $65K trade-in for the old ABI 3700 will make this request more cost effective. In addition, the Dean will support technician salaries after another grant terminates next year. With the requested ABI 3730xl DNA Analyzer, the DNAcore will continue to meet intense needs of our over 200 investigators with over 250 NIH grants for economical and accessible on-site sequencing and genotyping services to speed gene discovery, gene structure analysis, mutation characterization, analysis of vector constructs, linkage analysis, genome scanning, LOH studies, etc. In summary, the requested equipment upgrade will fit synergistically with existing resources and our Dean's major genomics initiatives to enhance research productivity by reducing costs of these key services to investigator's grant funds and providing higher quality sequencing and hitherto unavailable analyses to support high-throughput genomic scans.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：本NCRR共享仪器资助申请的总体目标是使用ABI 3730 xl DNA分析仪升级和更新大量使用的机构共享DNA测序和基因分型核心（DNAcore），以1）降低DNA测序和基因分型的成本，2）增加测序读段长度和质量，3）提供用于SNP分析的新的高通量ABI SNRISE方法。所要求的ABI 3730 xl DNA分析仪是目前最先进的毛细管阵列分析仪，将实现所有这些目标，并提供所需的经济性和灵活性，以满足需要我们共享资源设施的众多用户项目。对于我们从事基因关联项目的研究人员来说非常重要的是，它将利用各种方法支持低到高通量基因分型，包括基于STR的连锁，单SNP分析，SNaPshot测定的等位基因定量，以及48-plex SNP测定的高通量基因分型。此次对西奈山忙碌繁忙的共享测序、基因分型和寡核苷酸合成核心设施的升级将取代老化的ABI 3700平台，该平台运行成本太高，原因是试剂使用量大、硬件故障导致停机、无法支持SNAPIs检测以及相对于新ABI 3730 xl的信号灵敏度和均匀性降低。这一应用得到了其首席研究员过去10年的经验的加强，为西奈山迅速增加的NIH和其他研究人员提供高质量的DNA测序，基因分型，突变检测和寡核苷酸合成。DNAcore为所有测序、基因分型、定位克隆、突变检测和洛缺失项目提供内部专业知识和咨询服务。将通过DNAcore的地方咨询委员会促进公平、具有成本效益和顺应需要地利用所要求的仪器。他们还将确定和推荐新的战略，技术，仪器和资源，以进一步促进和促进前沿研究。研究院院长将支持服务合同，并提供10万美元用于购买所请求的ABI 3730 xl，其中6.5万美元用于旧ABI 3700的折价将使该请求更具成本效益。此外，院长将支持技术人员的工资后，另一个赠款终止明年。随着ABI 3730 xl DNA分析仪的需求，DNAcore将继续满足我们200多名研究人员的强烈需求，并获得250多项NIH赠款，用于经济和方便的现场测序和基因分型服务，以加速基因发现，基因结构分析，突变表征，载体构建分析，连锁分析，基因组扫描，洛缺失研究等。所要求的设备升级将与现有资源和我们院长的主要基因组学倡议协同配合，以通过降低这些关键服务的成本来提高研究生产力，以研究者的赠款基金，并提供更高质量的测序和迄今为止无法获得的分析，以支持高通量基因组扫描。