ABI 3730XL DNA ANALYZER: BREAST CANCER, AGING AND CANCER, CARCINOMA

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：这项NCRR共享仪器资助申请的总体目标是升级和更新使用频繁的机构共享DNA测序和基因分型核心(DNAcore)和ABI 3730xl DNA分析仪，以1)降低DNA测序和基因分型的成本，2)增加测序读取长度和质量，以及3)为SNP分析提供新的高通量ABI SNPlex方法。所要求的ABI 3730xl DNA分析仪是目前最先进的毛细管阵列分析仪，它将实现所有这些目标，并提供所需的经济性和灵活性，以满足需要我们共享资源设施的众多用户项目。对于我们从事基因关联项目的研究人员来说，它非常重要，它将支持利用各种方法进行低通量到高通量的基因分型，包括基于STR的连锁、单SNP分析、快照分析的等位基因定量，以及48-plex SNPlex分析的高通量基因分型。我们位于西奈山繁忙的共享测序、基因分型和寡核苷酸合成核心设施的这次升级将取代老化的ABI 3700平台，该平台由于试剂使用量大得多、硬件故障导致的停机时间、无法支持SNPlex分析以及与新的ABI 3730xl相比信号灵敏度和一致性降低而导致运行成本过高。其首席调查员在为西奈山迅速增加的NIH和其他研究人员提供高质量DNA测序、基因分型、突变检测和寡核苷酸合成方面的前10年经验加强了这一应用。DNAcore为所有测序、基因分型、位置克隆、突变检测和杂合性缺失项目提供内部专业知识和咨询服务。将通过DNAcore的地方咨询委员会促进公平、成本效益高和反应迅速地利用所要求的仪器。他们还将确定和推荐新的战略、技术、仪器和资源，以进一步促进和促进尖端研究。研究主任将支持服务合同，并为购买所需的ABI 3730xl提供10万美元，再加上旧ABI 3700的6.5万美元折价，将使这一请求更具成本效益。此外，在明年另一笔拨款终止后，院长将支持技术员的工资。有了所要求的ABI 3730xl DNA分析仪，DNAcore将继续满足我们200多名研究人员的强烈需求，他们拥有超过250项NIH拨款，用于经济和可获得的现场测序和基因分型服务，以加快基因发现、基因结构分析、突变表征、载体构建分析、连锁分析、基因组扫描、杂合性研究等。总而言之，所要求的设备升级将与现有资源和我们院长的主要基因组学计划协同配合，通过降低这些关键服务的成本来提高研究生产率，为研究人员的赠款资金，并提供更高质量的测序和迄今无法提供的分析，以支持高通量基因组扫描。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The overall objective of this NCRR Shared Instrument Grant application is to upgrade and update a heavily-used Institutional Shared DNA Sequencing and Genotyping Core (DNAcore) with an ABI 3730xl DNA Analyzer to 1) lower costs of DNA sequencing and genotyping, 2) increase sequencing read-length and quality, and to 3) provide the new high-throughput ABI SNPlex method for SNP analysis. The requested ABI 3730xl DNA Analyzer is the current state-of-the-art capillary array analyzer that will fulfill all these goals and provide the economy and flexibility needed to satisfy the multitude of user projects requiring our shared resource facility. Of great importance to our investigators working on gene association projects, it will support low to high throughput genotyping utilizing a wide variety of approaches including STR-based linkage, single SNP analyses, allele quantitation by SNaPshot assays, and high throughput genotyping with 48-plex SNPlex assays. This upgrade to our busy shared sequencing, genotyping and oligonucleotide synthesis core facility at Mount Sinai will replace the ageing ABI 3700 platform that is too expensive to run due to its much greater use of reagents, its downtime due to hardware failures, its inability to support the SNPlex assay and its reduced signal sensitivity and uniformity relative to the new ABI 3730xl. This application is strengthened by the previous 10 years of experience of its Principal Investigator in providing high-quality DNA sequencing, genotyping, mutation detection and oligonucleotide synthesis for rapidly increasing numbers of NIH and other investigators at Mount Sinai. The DNAcore provides in-house expertise and consultation services for all sequencing, genotyping, positional cloning, mutation detection, and LOH projects. Equitable, cost-effective and responsive utilization of the requested instrumentation will be promoted through the DNAcore's Local Advisory Committee. They will also identify and recommend novel strategies, technologies, instrumentation, and resources to further facilitate and foster cutting-edge research. The Dean for Research will support service contracts and provide $100K towards the purchase of the requested ABI 3730xl, which with the $65K trade-in for the old ABI 3700 will make this request more cost effective. In addition, the Dean will support technician salaries after another grant terminates next year. With the requested ABI 3730xl DNA Analyzer, the DNAcore will continue to meet intense needs of our over 200 investigators with over 250 NIH grants for economical and accessible on-site sequencing and genotyping services to speed gene discovery, gene structure analysis, mutation characterization, analysis of vector constructs, linkage analysis, genome scanning, LOH studies, etc. In summary, the requested equipment upgrade will fit synergistically with existing resources and our Dean's major genomics initiatives to enhance research productivity by reducing costs of these key services to investigator's grant funds and providing higher quality sequencing and hitherto unavailable analyses to support high-throughput genomic scans.