FEI Morgagni Transmission Electron Microscope

描述（由申请人提供）： 该提案要求为高通量透射电子显微镜（TEM）和相关配件提供资金，奥克兰大学眼科研究所、生物科学系和物理系的8个主要用户将利用共享设备研究人类疾病状态下的基本生物过程。FEIMorgagni TEM用于研究正在进行的生理和病理途径，它的易用性使其非常适合多用户的科学环境。需要并将广泛使用所要求设备的资助项目包括：1）正常和退化的内部视网膜神经元的信号转导，2）遗传性视网膜退化的基因治疗，3）细胞外基质在青光眼中神经细胞死亡中的作用，4）透镜上皮细胞再生和白内障形成的机制，5）视网膜变性的光感受器相关机制，6）健康和疾病中的视网膜代谢，7）人透镜上皮细胞系的建立，和8）正常和患病关节软骨中胶原纤维取向的测定所要求的仪器将使完成资助项目中所述目标所需的快速和容易的高质量图像采集和分析成为可能。一个咨询委员会，由四个主要用户组成，EM实验室主管将监督和协调仪器的使用和维护。设备将被安置在奥克兰大学眼科研究所内建立的电子显微镜实验室套件中，奥克兰大学（Oakland University）认识到对该仪器的迫切需求，并承诺提供适当的空间和财政支持，以支付持续的维护费用

DESCRIPTION (provided by applicant): This proposal requests funding for a high-throughput transmission electron microscope (TEM) and related accessories, eight major users in the Eye Research Institute, Department of Biological Sciences, and Physics Department of Oakland University will utilize the shared equipment for investigations into fundamental biological processes in human disease states The equipment requested, a FEI Morgagni TEM is intended for the study of ongoing physiological and pathological pathways, and its ease-of-use makes it superbly suited to a multi-user scientific environment Current NIH-funded projects that require and will make extensive use of the requested equipment include 1) signal transduction in normal and degenerative inner retinal neurons, 2) gene therapy for inherited retinal degenerations, 3) role of extracellular matrix in nerve cell death in glaucoma, 4) mechanisms of lens epithelia cell regeneration and cataract formation, 5) photoreceptor related mechanisms of retinal degeneration, 6) retinal metabolism in health and disease, 7) establishment of human lens epithelial cell lines, and 8) determination of collagen fiber orientation in normal and diseased articular cartilage The requested instrument will make possible rapid and facile high qualit) image acquisition and analysis required to complete aims described in the funded projects An advisory committee, consisting of four major users, and an EM laboratory supervisor will oversee and coordinate use and maintenance of the instrument The equipment will be housed in the established Electron Microscopy Laboratory Suite within the Eye Research Institute of Oakland University, the facility will be made available to other members of the campus community on an as-available basis, with priority given to NIH-funded projects Oakland University (OU) recognizes the immediate need for this instrument and has committed appropriate space and financial support for ongoing maintenance costs