FEI Morgagni Transmission Electron Microscope

描述(由申请人提供)： 这项提案要求为高通量透射电子显微镜(TEM)和相关附件提供资金，奥克兰大学眼科研究所、生物科学系和物理系的八个主要用户将利用共享设备研究人类疾病的基本生物过程。FEI Morgagni TEM用于研究正在进行的生理和病理途径，其易用性使其非常适合多用户科学环境当前NIH资助的项目需要并将广泛使用所需设备包括1)正常和变性视网膜内神经元的信号转导，2)遗传性视网膜变性的基因治疗，3)细胞外基质在青光眼神经细胞死亡中的作用，4)晶状体上皮细胞再生和白内障形成的机制，5)视网膜变性的光感受器相关机制，6)健康和疾病中的视网膜代谢，7)建立人类晶状体上皮细胞系，以及8)在正常和患病的关节软骨中确定胶原纤维方向所需的仪器将使快速和方便的高质量图像采集和分析成为可能，以完成资助项目中描述的目标一个由四个主要使用者组成的咨询委员会，一名EM实验室主管将监督和协调仪器的使用和维护设备将存放在奥克兰大学眼科研究所内已建立的电子显微镜实验室套件中，该设施将在可用的基础上提供给校园社区的其他成员，优先考虑NIH资助的项目奥克兰大学(OU)认识到对该仪器的迫切需求，并已承诺为持续维护费用提供适当的空间和财政支持

DESCRIPTION (provided by applicant): This proposal requests funding for a high-throughput transmission electron microscope (TEM) and related accessories, eight major users in the Eye Research Institute, Department of Biological Sciences, and Physics Department of Oakland University will utilize the shared equipment for investigations into fundamental biological processes in human disease states The equipment requested, a FEI Morgagni TEM is intended for the study of ongoing physiological and pathological pathways, and its ease-of-use makes it superbly suited to a multi-user scientific environment Current NIH-funded projects that require and will make extensive use of the requested equipment include 1) signal transduction in normal and degenerative inner retinal neurons, 2) gene therapy for inherited retinal degenerations, 3) role of extracellular matrix in nerve cell death in glaucoma, 4) mechanisms of lens epithelia cell regeneration and cataract formation, 5) photoreceptor related mechanisms of retinal degeneration, 6) retinal metabolism in health and disease, 7) establishment of human lens epithelial cell lines, and 8) determination of collagen fiber orientation in normal and diseased articular cartilage The requested instrument will make possible rapid and facile high qualit) image acquisition and analysis required to complete aims described in the funded projects An advisory committee, consisting of four major users, and an EM laboratory supervisor will oversee and coordinate use and maintenance of the instrument The equipment will be housed in the established Electron Microscopy Laboratory Suite within the Eye Research Institute of Oakland University, the facility will be made available to other members of the campus community on an as-available basis, with priority given to NIH-funded projects Oakland University (OU) recognizes the immediate need for this instrument and has committed appropriate space and financial support for ongoing maintenance costs