Healthy ageing in DNA

DNA 中的健康老龄化

• 批准号: 2368337
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2368337
• 关键词: Healthy; ageing; DNA

In this project the student will use variant calling from single cell sequencing studies to identify the amount and character of mutations in single cells with age. Working with experts in senescence (Gil) and in mutation through development and ageing (Chinnery) the student will explore how mutations accumulate in single cells and whether they might have a phenotypic effect consonant with ageing. Datasets will include those studying single cell senescence, development and ageing and we will draw on both genome sequencing studies and scRNA-Seq data. When candidate sets of genes are identified we will use informatics tools to identify the molecular pathways which are modulated by different kinds of mutation. This builds on pre-existing work in the lab of Nick Jones developing an DNA variant calling pipeline and testing it on the ageing pancreas and eye.

在这个项目中，学生将使用单细胞测序研究中的变异识别来确定单细胞中随年龄变化的突变数量和特征。与衰老（Gil）和通过发育和衰老（Chinnery）突变的专家合作，学生将探索突变如何在单细胞中积累，以及它们是否可能具有与衰老一致的表型效应。数据集将包括研究单细胞衰老，发育和衰老的数据集，我们将利用基因组测序研究和scRNA-Seq数据。当候选基因集被确定后，我们将使用信息学工具来确定由不同类型的突变调节的分子途径。这是建立在尼克·琼斯（Nick Jones）实验室先前的工作基础上的，他开发了一种DNA变体调用管道，并在衰老的胰腺和眼睛上进行了测试。