Core--Nuclear Magnetic Resonance Facility

核心——核磁共振装置

• 批准号: 6990230
• 负责人: Stephen M Prescott
• 金额: $ 1.65万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-09 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990230
• 关键词: biomedical facility; neoplasm /cancer; nuclear magnetic resonance spectroscopy

The Biomolecular Nuclear Magnetic Resonance (NMR) Shared Resource provides a state-of-the-art laboratory for biomolecular structure determination as well as routine analytical NMR services for the health sciences research community. It currently has three NMR spectrometers operating at 400, 500, and 600 MHz. The 500 MHz spectrometer will be upgraded in 2003 with all new electronics (the "console"). Upon upgrade of the 500 MHz machine, both the 500 and 600 MHz spectrometers will be state-of-the-art for biomolecular NMR, with 3 radiofrequency channels plus 2H decoupling. The 600 MHz spectrometer (Varian Inova) is the highest field NMR spectrometer in the state of Utah. The 400 MHz spectrometer (Varian Mercury) is dedicated to small molecule, organic and natural products NMR applications, and is exceptionally versatile and easy to use. The NMR facility also has several Sun and Silicon Graphics Inc. workstations for offline data processing and biomolecular structure determination. These computers are part of the Structural Biology Computing Center. Software on the SGIs includes VNMR, Insight/Discover, Felix, NMRpipe, XEASY, SPARKY, MOLMOL, CNS, XPLOR and DYANA. The resource is supervised by two faculty directors with established research programs that utilize NMR. In addition, a Ph.D.-level resource manager is responsible for technical support for the most demanding protein and RNA structure determination projects. This manager is responsible for implementing new heteronuclear 3D and 4D NMR experiments for biomolecular NMR, and assisting researchers with data collection as well as data processing and analysis. The resource also has a full-time NMR technician responsible for running small-molecule NMR experiments on the 400 MHz and 500 MHz spectrometers. The NMR technician also trains new users of the NMR spectrometers and performs routine maintenance.

生物分子核磁共振（NMR）共享资源为生物分子结构测定提供了最先进的实验室，并为健康科学研究社区提供常规分析NMR服务。它目前有三台NMR光谱仪，工作频率分别为400、500和600 MHz。 500兆赫频谱仪将于2003年升级，配备所有新的电子设备（“控制台”）。500 MHz仪器升级后，500 MHz和600 MHz光谱仪将成为生物分子NMR的最新技术，具有3个射频通道和2 H去耦。600 MHz光谱仪（Varian Inova）是犹他州最高的场NMR光谱仪。400 MHz光谱仪（Varian Mercury）专用于小分子、有机和天然产物NMR应用，用途广泛且易于使用。核磁共振设施也有几个太阳和硅图形公司。用于离线数据处理和生物分子结构测定的工作站。这些计算机是结构生物学的一部分 计算中心。SGI上的软件包括VNMR、Insight/Discover、Felix、NMRpipe、XEASY、SPARKY、MOLMOL、CNS、XPLOR和DYANA。该资源由两名教师主任监督，他们拥有利用NMR的既定研究计划。此外，博士学位-一级资源经理负责为最苛刻的蛋白质和RNA结构测定项目提供技术支持。该经理负责实施生物分子NMR的新的异相3D和4D NMR实验，并协助研究人员进行数据收集以及数据处理和分析。该资源还拥有一名全职核磁共振技术员，负责在400台 MHz和500 MHz光谱仪。NMR技术员还培训NMR光谱仪的新用户并进行日常维护。