Core--Microarray Facility

核心——微阵列设备

• 批准号: 6990228
• 负责人: Stephen M Prescott
• 金额: $ 2.93万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-09 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990228
• 关键词: biomedical facility; genetic library; genetic registry /resource /referral center; microarray technology; molecular biology information system; neoplasm /cancer

The Microarray Shared Resource provides microarray services to members of the Cancer Center as well as to other University investigators. These services include printing of microarray slides, analysis of samples on an Agilent BioAnalyzer to define RNA quality, labeling of samples with Cy3 and Cy5 fluorescent dyes, microarray hybridization, microarray scanning and data capture, data analysis, and cDNA clone distribution. The facility has acquired several key pieces of instrumentation including an Amersham Lucidea Array Spotter, an Axon 4000B Scanner, an Agilent BioAnalyzer, two BioMicro Systems MAUI hybridization units, a Packard Instruments Multiprobe II liquid handler, and an MJ Research Tetrad thermal cycler. Scanned arrays are quantified using BioDiscovery ImaGene software; GeneSight software is available to clients of the Microarray Resource for gene mining and data analysis of their arrays. A microarray laboratory information management system is used to track clones, slides, workflow, samples, experiment requests and data. The Microarray Resource has acquired an extensive collection of cDNA clones that includes human (42,000), mouse (34,000), yeast ORFs (6,000), yeast intergenic sequences (6,000), Drosophila (12,000), Planaria (4,000) and newt (1,500). On an annual basis, approximately 2000 microarray analyses are performed. The Microarray Resource proposes to enhance services in the following areas: expansion of the current clone collections, enhancement of microarray data storage capability, and increased levels of support for microarray data analysis and interpretation. To expand our ability to offer microarrays derived from experimental organisms actively used in cancer studies, we hope to acquire a zebrafish oligo library collection. To improve data storage capabilities of the Microarray Resource, we plan to acquire an Iomega NAS P850m data storage server (funding requested in the Informatics Shared Resource). An additional area of emphasis at the facility will be to provide consultation in experimental design, analysis and interpretation of data. To accomplish this goal, we have added a staff member to focus on consulting with our clients in these areas.

微阵列共享资源为癌症中心的成员以及其他大学研究人员提供微阵列服务。这些服务包括微阵列载玻片的打印，在Agilent BioAnalyzer上分析样品以确定RNA质量，用Cy 3和Cy 5荧光染料标记样品，微阵列杂交，微阵列扫描和数据捕获，数据分析和cDNA克隆分布。 该设施已经获得了几个关键的仪器，包括一个阿默舍姆Lucidea阵列点样仪，一个Axon 4000 B扫描仪，一个Agilent BioAnalyzer，两个BioMicro Systems MAUI杂交单元，一个Packard Instruments Multiprobe II液体处理器和一个MJ Research Tetrad热循环仪。使用BioDiscovery ImaGene软件对扫描的阵列进行定量; GeneSight软件可供Microarray Resource的客户用于其阵列的基因挖掘和数据分析。微阵列实验室信息管理系统用于跟踪克隆、载玻片、工作流程、样品、实验请求和数据。微阵列资源已经获得了大量的cDNA克隆，包括人类（42，000），小鼠（34，000），酵母ORF（6，000），酵母基因间序列（6，000），果蝇（12，000），Planaria （4，000）和蝾螈（1，500）。每年进行大约2000次微阵列分析。微阵列资源建议在以下领域加强服务：扩大目前的克隆收藏，提高微阵列数据存储能力，并提高对微阵列数据分析和解释的支持水平。为了扩大我们提供来自癌症研究中积极使用的实验生物的微阵列的能力，我们希望获得斑马鱼寡核苷酸文库。为了提高微阵列资源的数据存储能力，我们计划购买一台Iomega NAS P850 m数据存储服务器（在信息学共享资源中申请资金）。该设施的另一个重点领域将是在实验设计、分析和数据解释方面提供咨询。为了实现这一目标，我们增加了一名工作人员，专注于与这些领域的客户进行咨询。