Core D: Bone Cell Core

虽然标准技术是可用的，但实验室内的质量和 耶鲁大学用于研究目的的骨细胞数量变化很大。到 解决这一限制，并推进生产性奖学金，耶鲁核心中心为 肌肉骨骼疾病发展了细胞核心。细胞核心的使命是 也有利于真实的、原代的、小鼠和大鼠成骨细胞的实验使用 如体外培养产生的破骨细胞。 作为我们新举措的一部分，骨髓间充质干细胞（MSC）现在 提供给核心研究者。虽然不属于核心的最初任务范围， 导演通过协议向中心成员提供他的PIXImus机器 生理学核心的密度测量子单元 目前建议的具体目标是提供以下服务： 1.原代分离培养小鼠和胎鼠颅骨成骨细胞。这将包括 用于生物化学研究以及RNA和DNA纯化的大规模制备。 2.培养小鼠破骨细胞样细胞，其产生自：1）体外共培养2）与M-CSF和RANKL的培养。 3.保持广泛的冷冻细胞系。 4.提供小动物骨密度测定（DEXA）。 5.培养小鼠骨髓间充质干细胞。 6.体外分离培养成骨细胞、破骨细胞和基质细胞， 经过适当培训的研究人员可以在其实验室启动和维持这些细胞。 细胞核心特别渴望支持当前和未来的试点和可行性项目，作为该中心正在努力吸引新的研究人员进行肌肉骨骼研究的一部分。

Although standard techniques are available, intra-laboratory variation in the quality and number of bone cells available for research purposes at Yale has varied considerably. To address this limitation and to advance productive scholarship, the Yale Core Center for Musculoskeletal Disorders developed the Cell Core. The mission of the Cell Core is to facilitate the experimental use of authentic, primary, mouse and rat osteoblastic cells as well as osteoclasts generated from in vitro culture. As part of our new initiatives bone marrow derived mesenchymal stem cells (MSCs) are now available to Core investigators. Although outside the original mandate of the Core, the Director has made his PIXImus machine available to Center members through an agreement with the Densitometry subunit of the Physiology Core. The Specific Aims of the current proposal are to provide the following services: 1. Isolate and cultivate primary murine and fetal rat calvarial osteoblasts. This will include large-scale preparations for biochemical studies and RNA and DNA purification. 2. Cultivate murine osteoclast-like cells generated from: 1) in vitro co-culture 2) culture with M-CSF and RANKL. 3. Maintain a wide range of frozen cell lines. 4. Provide small animal bone densitometry (DEXA). 5. Cultivate mouse bone marrow derived mesenchymal stem cells (MSCs). 6. Train for in vitro isolation and culture of osteoblasts, osteoclasts and stromal cells, so that investigators, appropriately trained, can initiate and maintain these cells in their laboratories. The Cell Core is particularly anxious to support current and future Pilot and Feasibility Projects as part of the Center's ongoing effort to attract new investigators to musculoskeletal research.