Cell Core

虽然有标准技术可用，但实验室内质量和 耶鲁大学可用于研究目的的骨细胞数量差异很大。至 为了解决这一限制并推进生产性学术研究，耶鲁大学核心研究中心 肌肉骨骼疾病形成了细胞核心。细胞核心的使命是 促进真实、原代、小鼠和大鼠成骨细胞的实验使用 作为体外培养产生的破骨细胞。 作为我们新倡议的一部分，骨髓间充质干细胞(MSCs)现在 可供核心调查人员使用。虽然超出了核心的最初任务，但 主任已经通过一项协议向中心成员提供了他的PIXImus机器 生理学核心的密度测量子单元。 目前提案的具体目的是提供以下服务： 1.分离培养原代小鼠和胎鼠颅骨成骨细胞。这将包括 用于生化研究和RNA和DNA提纯的大规模准备。 2.小鼠破骨样细胞的培养：1)体外共培养2)MCSF 和RANKL。 3.保持广泛的冷冻细胞系。 4.提供小动物骨密度测量(DEXA)。 5.培养小鼠骨髓间充质干细胞。 6.培训成骨细胞、破骨细胞和基质细胞的体外分离和培养，以便 经过适当培训的研究人员可以在他们的实验室中启动和维护这些细胞。 这些服务在马克·霍洛维茨博士的指导下进行协调，他曾 在一般细胞培养和骨细胞分离培养方面有丰富的经验 具体地说。CELL核心特别渴望支持当前和未来的试点和 可行性项目是该中心吸引新调查人员的持续努力的一部分 肌肉骨骼研究。 演出现场(S)(组织、词组

Although standard techniques are available, intra-laboratory variation in the quality and number of bone cells available for research purposes at Yale has varied considerably. To address this limitation and to advance productive scholarship, the Yale Core Center for Musculoskeletal Disorders developed the Cell Core. The mission of the Cell Core is to facilitate the experimental use of authentic, primary, mouse and rat osteoblastic cells as well as osteoclasts generated from in vitro culture. As part of our new initiatives bone marrow derived mesenchymal stem cells (MSCs) are now available to Core investigators. Although outside the original mandate of the Core, the Director has made his PIXImus machine available to Center members through an agreement with the Densitometry subunit of the Physiology Core. The Specific Aims of the current proposal are to provide the following services: 1. Isolate and cultivate primary murine and fetal rat calvarial osteoblasts. This will include large-scale preparations for biochemical studies and RNA and DNA purification. 2. Cultivate murine osteoclast-like cells generated from: 1) in vitro co-culture 2) culture with MCSF and RANKL. 3. Maintain a wide range of frozen cell lines. 4. Provide small animal bone densitometry (DEXA). 5. Cultivate mouse bone marrow derived mesenchymal stem cells (MSCs). 6. Train for in vitro isolation and culture of osteoblasts, osteoclasts and stromal cells, so that investigators, appropriately trained, can initiate and maintain these cells in their laboratories. These services are coordinated under the directorship of Dr. Mark Horowitz, who has had extensive experience with cell culture in general and the isolation and culture of bone cells specifically. The Cell Core is particularly anxious to support current and future Pilot and Feasibility Projects as part of the Center's ongoing effort to attract new investigators to musculoskeletal research. PERFORMANCE SITE(S) (organization, ci