Animal Research Core

动物研究核心

• 批准号: 6794556
• 负责人: RONALD GARY TOMPKINS
• 金额: $ 3.34万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6794556
• 关键词: biomedical facility; burns; disease /disorder model; genetically modified animals; laboratory mouse; laboratory rabbit; metabolism; model design /development; trauma

The Animal Research Core examines changes in metabolic patterns and evaluates the cellular and molecular changes in organs, blood, and tissues from mice and rabbits following established mouse and rabbit models of burn injury that recapitulate the development of the hypermetabolic response from the Human Subjects Core. Knowledge acquired from observation of the common changes in the metabolic response after burn injury will build upon our strong foundation for comparisons between metabolic changes observed in the clinical samples and animal studies. The interpretation of the results from the murine and rabbit models can help us redesign our models to more closely mimic the human phenotype. Such studies are required for the development of effective therapeutic strategies to prevent the occurrence of potentially fatal complications in seriously burned patients. This facility ensures that all investigation perform the burn injury with uniform, reproducible results. Secondly, we strive to have all murine studies including the knockout models on a C57BL/6 background to facilitate interpretation and comparison of our results. Preparation and handling of the complex animal models used in the Burn Trauma Center can be problematic for young investigators and research fellows without substantial experience. The Animal Research Core is extremely helpful in the pre-and post-operative care and the administrative responsibilities for animal care, and has provided consistency and reproducibility within and between studies, while insuring that animal welfare is maintained. The Animal Research Core provides several services to the Burn Trauma Center. The core provides the investigators with a central facility and technical staff that is equipped to set up and maintain uniform, complex mouse and rabbit models. The facility assumes an enormous responsibility that all animal studies funded by the Center are current and up-to-date and that all procedures follow strict humane guidelines. The facility has acquired a library of books and protocols related to the performance of animal procedures and freely shares this information with Center investigators.

动物研究核心检查代谢模式的变化，并在建立小鼠和兔子烧伤模型后评估小鼠和兔子器官、血液和组织的细胞和分子变化，这些模型概括了人类受试者核心代谢亢进反应的发展。通过观察烧伤后代谢反应的常见变化而获得的知识将为我们在临床样本和动物研究中观察到的代谢变化之间的比较奠定坚实的基础。对小鼠和兔子模型结果的解释可以帮助我们重新设计模型，以更接近地模仿人类表型。需要进行此类研究 制定有效的治疗策略，以防止严重烧伤患者发生潜在致命的并发症。该设施确保所有烧伤调查都能获得统一、可重复的结果。其次，我们努力在 C57BL/6 背景上进行所有小鼠研究，包括敲除模型，以便于解释和比较我们的结果。对于没有丰富经验的年轻研究人员和研究人员来说，烧伤创伤中心使用的复杂动物模型的准备和处理可能会出现问题。动物研究核心在前期和后期非常有帮助 术后护理和动物护理的行政责任，并在研究内部和之间提供一致性和可重复性，同时确保维持动物福利。动物研究核心为烧伤创伤中心提供多项服务。该核心为研究人员提供了中央设施和技术人员，能够建立和维护统一、复杂的小鼠和兔子模型。该机构承担着巨大的责任，确保该中心资助的所有动物研究都是最新的、最新的，并且所有程序都遵循严格的人道准则。该设施已获得与动物程序执行相关的书籍和协议图书馆，并免费共享 向中心调查员提供信息。