Animal Research Core

动物研究核心

• 批准号: 6794556
• 负责人: RONALD GARY TOMPKINS
• 金额: $ 3.34万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6794556
• 关键词: biomedical facility; burns; disease /disorder model; genetically modified animals; laboratory mouse; laboratory rabbit; metabolism; model design /development; trauma

The Animal Research Core examines changes in metabolic patterns and evaluates the cellular and molecular changes in organs, blood, and tissues from mice and rabbits following established mouse and rabbit models of burn injury that recapitulate the development of the hypermetabolic response from the Human Subjects Core. Knowledge acquired from observation of the common changes in the metabolic response after burn injury will build upon our strong foundation for comparisons between metabolic changes observed in the clinical samples and animal studies. The interpretation of the results from the murine and rabbit models can help us redesign our models to more closely mimic the human phenotype. Such studies are required for the development of effective therapeutic strategies to prevent the occurrence of potentially fatal complications in seriously burned patients. This facility ensures that all investigation perform the burn injury with uniform, reproducible results. Secondly, we strive to have all murine studies including the knockout models on a C57BL/6 background to facilitate interpretation and comparison of our results. Preparation and handling of the complex animal models used in the Burn Trauma Center can be problematic for young investigators and research fellows without substantial experience. The Animal Research Core is extremely helpful in the pre-and post-operative care and the administrative responsibilities for animal care, and has provided consistency and reproducibility within and between studies, while insuring that animal welfare is maintained. The Animal Research Core provides several services to the Burn Trauma Center. The core provides the investigators with a central facility and technical staff that is equipped to set up and maintain uniform, complex mouse and rabbit models. The facility assumes an enormous responsibility that all animal studies funded by the Center are current and up-to-date and that all procedures follow strict humane guidelines. The facility has acquired a library of books and protocols related to the performance of animal procedures and freely shares this information with Center investigators.

动物研究核心检查代谢模式的变化，并评价建立烧伤小鼠和家兔模型后小鼠和家兔器官、血液和组织中的细胞和分子变化，该模型重现了人类受试者核心的高代谢反应的发展。从观察烧伤后代谢反应的常见变化中获得的知识将建立在我们对临床样本和动物研究中观察到的代谢变化进行比较的坚实基础上。对鼠和兔模型结果的解释可以帮助我们重新设计我们的模型，以更接近地模拟人类表型。这些研究是必要的， 制定有效的治疗策略，以防止严重烧伤患者发生潜在的致命并发症。该设施确保所有调查执行烧伤具有统一的，可重复的结果。其次，我们努力使所有的小鼠研究，包括C57 BL/6背景下的敲除模型，以便于解释和比较我们的结果。烧伤创伤中心使用的复杂动物模型的准备和处理对于没有丰富经验的年轻研究人员和研究人员来说可能是有问题的。动物研究核心是非常有帮助的， 术后护理和动物护理的管理职责，并在研究内和研究之间提供了一致性和再现性，同时确保动物福利得到维持。动物研究中心为烧伤创伤中心提供多种服务。核心为研究人员提供了一个中央设施和技术人员，配备了建立和维护统一的，复杂的小鼠和兔子模型。该机构承担着巨大的责任，即由该中心资助的所有动物研究都是最新的，所有程序都遵循严格的人道准则。该设施已经获得了一个图书馆的书籍和协议有关的性能的动物程序，并免费分享这一点 中心研究人员的信息。