Radio-labelled responsive fluorophores to elucidate the role of MMP in ageing

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Matrix metalloproteinases (MMPs) play an essential role in a variety of biological processes,including ageing. Within this project, a new platform of multifunctional probes for the detailedstudy of MMP's role in age-related diseases will be developed. The probes will consist of anorganic fluorophore covalently linked to a PET-tracer and anchored via an MMP cleavablepeptide sequence to a black-hole quencher (BHQ). The PET-tracer will allow for the spatialmonitoring of the probe's diffusion in the cellular matrix, while the BHQ will add temporalresolution when cleaved by MMP, thereby "turning-on" the fluorescence.