Telomerase re-expression in postmorterm CNS Progenitors.

死后中枢神经系统祖细胞中端粒酶的重新表达。

• 批准号: 6794018
• 负责人: Robert Mark Richardson
• 金额: $ 1.34万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6794018
• 关键词: Parkinson' s disease; apoptosis; cell differentiation; cell line; cell proliferation; cell type; central nervous system; gene expression; human tissue; laboratory rat; nervous system regeneration; neurons; postmortem; stem cell transplantation; stem cells; telomerase; transfection; transposon /insertion element; tyrosine 3 monooxygenase

DESCRIPTION (provided by applicant): Transplantation strategies using CNS stem cells offer tremendous potential for replacing neuronal circuitry lost to Parkinson?s disease and other neurological disorders. The ability of CNS grafts to survive after transplant is crucial if therapeutic benefit is to be provided to a large number of patients with PD. Additionally, there must be an adequate source of donor cells. The purpose of this proposal is to insert the human telomerase gene (hTERT) into human postmortem-derived neural progenitor cells (HPCPs) in order to improve the survival of transplanted cells and ultimately increase the proliferation of desired cell types. HPCPs provide an easily accessible donor source, and ectopic expression of hTERT is a logical means by which to immortalize these cells in culture and decrease their susceptibility to apoptotic death following transplantation. Determining the effect of ectopic telomerase expression in HPCPs on population doublings, resistance to apoptosis and ability to differentiate into all CNS cell types is the crucial first step in investigating these methods. Assessing the viability, proliferation and differential fates of hTERT+ HPCPs transplanted to a 6-OHDA lesioned rat model of Parkinson?s disease, and evaluating functional recovery in transplant recipients will further characterize the extent to which these approaches may contribute to future stem cell therapy for neurological disorders. For the treatment of Parkinson? disease, a future step may include combining these methods with strategies likely to induce a dopaminergic fate among a subset of these progenitors.

描述（申请人提供）：使用中枢神经系统的移植策略

项目成果

Ectopic telomerase expression inhibits neuronal differentiation of NT2 neural progenitor cells.

异位端粒酶表达抑制 NT2 神经祖细胞的神经元分化。

• DOI: 10.1016/j.neulet.2007.03.079
• 发表时间: 2007
• 期刊: Neuroscience letters
• 影响因子: 2.5
• 作者: Richardson,RMark;Nguyen,Binh;Holt,ShawnE;Broaddus,WilliamC;Fillmore,HelenL
• 通讯作者: Fillmore,HelenL

Progress in cerebral transplantation of expanded neuronal stem cells.

扩增神经元干细胞脑移植研究进展

• DOI: 10.3171/jns.2004.100.4.0659
• 发表时间: 2004
• 期刊: Journal of neurosurgery
• 影响因子: 4.1
• 作者: Richardson,RMark;Fillmore,HelenL;Holloway,KathrynL;Broaddus,WilliamC
• 通讯作者: Broaddus,WilliamC