Three-dimensional Molecular Pattern Recognition

描述(申请人提供)：细胞生物学的一个基本主题 以大分子络合物的结构-功能关系为中心 负责细胞活动。结构生物学的一个目标是获得一种 这些大分子机器的不同功能描述 在这些国家和其结构环境中，这是一个重要的问题。虽然有可能想象大的 大分子组装，亚细胞结构，甚至细胞 对于电子显微镜来说，得到的地图的分辨率限制最多 通常排除了对这些重建的直接高分辨率解释。 我们建议开发一种用于鉴定的分子模式识别系统 以及分子在复杂结构环境中的位置。我们 将集中精力研究简化的肌动蛋白系统--以及 微管相关组件。发展的紧密耦合 方法学及其在这些具有生物学意义的生物中的应用 将使用组件。这样，我们将立即获得有关以下方面的反馈 系统在实践中如何运行，同时提供对这些问题的有价值的见解 系统。对于每一步，计算机生成的模型系统也将被用作 发展和评估的工具。文档、教程和简单的 将开发可供使用的界面。

DESCRIPTION (provided by applicant): A fundamental theme in cell biology centers on the structure-function relationships of the macromolecular complexes responsible for cellular events. One goal of structural biology is to obtain a description of these macromolecular machines in their different functional states and in their structural environment. While it is possible to image large macromolecular assemblies, sub-cellular structures and even cells with the aid of electron microscopy, the resolution restrictions of the resulting maps most often precludes direct high-resolution interpretation of these reconstructions. We propose to develop a molecular pattern recognition system for identification and location of molecules in the context of complex structural environments. We will concentrate our efforts on simplified systems of actin- and microtubule-related assemblies. A tight coupling of the development of the methodology with applications to these biologically highly significant assemblies will be employed . This way we will obtain immediate feedback as to how the system performs in practice while providing valuable insight into those systems. For each step, computer-generated model systems will also be used as a tool for development and evaluation. Documentation, tutorials and an easy to-use interface will be developed.