Mechanism of Bacterial SMC Complex MukBEF

细菌SMC复合物MukBEF的作用机制

• 批准号: 6931544
• 负责人: VALENTIN V RYBENKOV
• 金额: $ 18.19万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6931544
• 关键词: DNA topoisomerases; adenosinetriphosphatase; bacterial genetics; bacterial proteins; cell cycle; chemical kinetics; chromatin; compression; computer simulation; conformation; electron microscopy; enzyme substrate complex; genetic regulation; genetic regulatory element; hydrolysis; immunomagnetic separation; intermolecular interaction; model design /development; nucleic acid metabolism; nucleic acid structure; nucleotide analog; operon; physical model; protein structure function; southern blotting; structural biology

DESCRIPTION (provided by applicant): Chromatin structure undergoes marked, tightly controlled changes during the cell cycle. Maintaining proper chromatin structure is essential for faithful execution of such fundamental events as chromosome replication, recombination, gene expression or cell division. Errors in either of the processes often result in genomic instability and chromosomal alterations potentially leading to carcinogenesis or developmental defects. However molecular mechanisms underlying chromatin dynamics remain largely unknown. The SMC protein family is an emerging group of cellular ATPases responsible for large-scale chromatin reorganizations in organisms ranging from bacteria to humans. These proteins have been implicated in such diverse range of cellular functions as chromosome cohesion and condensation, DNA repair and dosage compensation. It was found recently that the mechanism of 13S condensin, a Xenopus SMC complex responsible for the chromosome condensation during cell division, involves direct ATP-dependent deformation of the large-scale DNA structure. This is an entirely novel kind of enzymatic activity. This project focuses on further mechanistic characterization of the SMC proteins using MukBEF, a bacterial analog of 13S condensin, as a model protein. The specific aims of this project are: (1) characterize biochemical activities of MukBEF; (2) investigate cell cycle regulation of MukBEF; (3) investigate the architecture of MukBEF-DNA complex using electron microscopy; (4) investigate DNA deformation by MukBEF using single DNA manipulation technique. These studies are expected to yield a comprehensive scheme of the MukBEF-catalyzed reaction. These data will contribute to our understanding of the intracellular functions of MukBEF and will illuminate the role of SMC proteins in large scale chromatin dynamics.

描述（由申请人提供）：染色质结构经过标记，

项目成果

MukEF Is required for stable association of MukB with the chromosome.

MukEF 是 MukB 与染色体稳定关联所必需的。

• DOI: 10.1128/jb.00770-07
• 发表时间: 2007
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: She,Weifeng;Wang,Qinhong;Mordukhova,ElenaA;Rybenkov,ValentinV
• 通讯作者: Rybenkov,ValentinV