Cbl-b in T Cell Activation and Autoimmunity

Cbl-b 在 T 细胞激活和自身免疫中的作用

• 批准号: 6821748
• 负责人: JIAN ZHANG
• 金额: $ 31.22万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-24 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6821748
• 关键词: CD28 molecule; SDS polyacrylamide gel electrophoresis; T cell receptor; T lymphocyte; antigen presentation; antigen receptors; autoimmunity; binding proteins; clinical research; genetically modified animals; human tissue; laboratory mouse; leukocyte activation /transformation; phosphorylation; ubiquitin

DESCRIPTION (provided by applicant): Autoimmune diseases result from the failure of the immune system to develop tolerance to self-proteins. The signaling threshold of antigen receptors and costimulatory receptors determine immunity or tolerance to self-proteins. A major costimulatory receptor, CD28, is required for induction of autoimmunity in several mouse models. CD28 costimulation amplifies early and late T cell receptor (TCR)-mediated signaling events. It has been shown that blocking CD28-mediated costimulation may represent one potential immunotherapy against autoimmunity. However, the molecular mechanisms that maintain immune tolerance to self-antigen in vivo and integrate costimulatory signals with the TCR signals are poorly understood. Recent studies suggest that CD28 costimulation may lower the threshold needed for T cell activation. Casitas-B-lineage lymphoma-b protein (Cbl-b), an adaptor protein and E3 ubiquitin ligase, can control CD28-dependent T cell activation via the regulation of Vav activity, suggesting a critical role of Cbl-b in the regulation of T cell activation threshold and hence in the maintenance of a balance between immunity and tolerance. Mice lacking Cbl-b spontaneously develop autoimmunity or have an increased susceptibility to experimental allergic encephalomyelitis. Therefore, Cbl-b may set the threshold for T cell activation. Our preliminary experiments showed that stimulation with higher doses of anti-CD3 partially overcomes defective T cell proliferation in CD28-deficient mice. Moreover, the presence or absence of CD28 may control TCR-induced Cbl-b ubiquitination and degradation. These findings provide a novel molecular mechanism(s) for how CD28 costimulation mediates optimal T cell activation. Based upon the above data, we hypothesize that Cbl-b regulates CD28-mediated T cell activation. Specifically, we will investigate whether and how CD28costimulation controls ubiquitination of Cbl-b; whether Cbl-b regulates CD28-mediated formation of immunological synapse; and whether Cbl-b regulates CD28-dependent autoreactive T cell activation in autoimmune arthritis. The information generated by the proposed studies will lead to a better understanding of Cbl-b in T cell biology and in autoreactive T cell responses, and will shed light on the development of novel therapeutic approaches to autoimmune arthritis.

描述（由申请人提供）：自身免疫性疾病是由于免疫系统未能对自身蛋白产生耐受性而引起的。抗原受体和共刺激受体的信号阈值决定了对自身蛋白的免疫或耐受。在几种小鼠模型中，诱导自身免疫需要一种主要的共刺激受体CD 28。CD 28共刺激放大早期和晚期T细胞受体（TCR）介导的信号传导事件。已经表明，阻断CD 28介导的共刺激可能代表一种针对自身免疫的潜在免疫疗法。然而，维持体内对自身抗原的免疫耐受以及整合共刺激信号与TCR信号的分子机制知之甚少。最近的研究表明，CD 28共刺激可能会降低T细胞活化所需的阈值。Casitas-B-lineage lymphoma-b protein（Cbl-b）是一种衔接蛋白和E3泛素连接酶，通过调节Vav活性来控制CD 28依赖性T细胞活化，提示Cbl-b在调节T细胞活化阈值，从而维持免疫和耐受之间的平衡中起着关键作用。缺乏Cbl-b的小鼠自发地发展自身免疫或对实验性过敏性脑脊髓炎的易感性增加。因此，Cbl-b可以设定T细胞活化的阈值。我们的初步实验表明，用更高剂量的抗CD 3刺激部分克服了CD 28缺陷小鼠中有缺陷的T细胞增殖。此外，CD 28的存在或不存在可以控制TCR诱导的Cbl-b泛素化和降解。这些发现为CD 28共刺激如何介导最佳T细胞活化提供了一种新的分子机制。基于上述数据，我们假设Cbl-b调节CD 28介导的T细胞活化。具体来说，我们将研究是否以及如何CD 28共刺激控制泛素化的Cbl-b的;是否Cbl-b调节CD 28介导的免疫突触的形成;以及是否Cbl-b调节CD 28依赖自身反应性T细胞激活自身免疫性关节炎。拟议研究产生的信息将导致更好地了解Cbl-b在T细胞生物学和自身反应性T细胞反应中的作用，并将揭示自身免疫性关节炎新治疗方法的发展。