Function of group III mGluRs at the calyx of Held

描述(申请人提供)：梯形体体内侧核是双耳声音定位回路中重要的符号倒置突触。MNTB内的主要突触是Hold，A。谷氨酸能轴体突触，具有独特的大突触前终末。这种形态允许成对的突触前和突触后细胞内记录，允许实时测量突触前囊泡释放和突触后受体反应。花萼表现出活性依赖的功能性突触可塑性，也经历了典型的胚胎后发育变化。有几个调节性递质和受体存在于Hold的花萼中，它们可能在发育过程中对联会属性的形成起到作用。其中，代谢型谷氨酸受体(MGluRs)是一个谜，因为它们迄今还没有显示出在突触可塑性或突触发育中明确的生理作用。我感兴趣的是研究Ill mGluRs组的功能作用，该组位于突触前Hold的花冠。结合免疫组织化学、电生理学和小鼠遗传学，我将确定Ill mGluRs组在突触可塑性和花瓣发育中的身份和作用。

DESCRIPTION (provided by applicant): The medial nucleus of the trapezoid body is an essential sign-inverting synapse in the binaural sound localization circuit. The major synapse in the MNTB is the calyx of Held, a. glutamatergic axosomatic synapse, with a uniquely large presynaptic terminal. This morphology allows paired pre-and postsynaptic intracellular recordings, allowing real-time measurements of presynaptic vesicle release, and postsynaptic receptor responses. The calyx shows activity-dependent functional synaptic plasticity, and also undergoes well-characterized postembryonic developmental changes. There are several modulatory transmitters and receptors present at the calyx of Held, that likely play a role in shaping the synpatic properties during development. 0f these, the metabotropic glutamate receptors, (mGluRs) are enigmatic, as they do not to date show a well-defined physiological role in either synaptic plasticity, or synaptic development. My interest lies in investigating the functional role of the group Ill mGluRs, which are located presynaptically at the calyx of Held. Using a combination of immunohistochemistry, electrophysiology, and murine genetics, I will determine the identity and role of group Ill mGluRs in synaptic plasticity and development of the calyx.