Function of group III mGluRs at the calyx of Held

描述（申请人提供）：梯形体内侧核是双耳声音定位回路中必不可少的信号反转突触。MNTB的主要突触是谷氨酸能轴体细胞突触的花萼，具有独特的大突触前末端。这种形态允许配对突触前和突触后的细胞内记录，允许实时测量突触前囊泡释放和突触后受体反应。花萼表现出活动依赖的功能性突触可塑性，并经历了典型的胚胎后发育变化。在花萼中存在几种调节递质和受体，它们可能在发育过程中形成突触特性。其中，代谢性谷氨酸受体（mGluRs）是一个谜，因为迄今为止它们在突触可塑性或突触发育中都没有明确的生理作用。我的兴趣在于研究il - mGluRs群的功能作用，它位于突触前的Held花萼。结合免疫组织化学、电生理学和小鼠遗传学，我将确定I组mGluRs在突触可塑性和花萼发育中的身份和作用。

DESCRIPTION (provided by applicant): The medial nucleus of the trapezoid body is an essential sign-inverting synapse in the binaural sound localization circuit. The major synapse in the MNTB is the calyx of Held, a. glutamatergic axosomatic synapse, with a uniquely large presynaptic terminal. This morphology allows paired pre-and postsynaptic intracellular recordings, allowing real-time measurements of presynaptic vesicle release, and postsynaptic receptor responses. The calyx shows activity-dependent functional synaptic plasticity, and also undergoes well-characterized postembryonic developmental changes. There are several modulatory transmitters and receptors present at the calyx of Held, that likely play a role in shaping the synpatic properties during development. 0f these, the metabotropic glutamate receptors, (mGluRs) are enigmatic, as they do not to date show a well-defined physiological role in either synaptic plasticity, or synaptic development. My interest lies in investigating the functional role of the group Ill mGluRs, which are located presynaptically at the calyx of Held. Using a combination of immunohistochemistry, electrophysiology, and murine genetics, I will determine the identity and role of group Ill mGluRs in synaptic plasticity and development of the calyx.