Mechanisms of 3'UTR Control: Yeast PUF Proteins
Mechanisms of 3'UTR Control: Yeast PUF Proteins
批准号:
6703657
负责人:
Aaron Charles Goldstrohm
金额:
$4.73万
依托单位国家:
美国
项目类别:
财政年份:
2003
资助国家:
美国
项目状态:
已结题
起止时间:
2003-02-01 至 2005-01-31
中文摘要
点击翻译按钮获取中文摘要
英文摘要
DESCRIPTION (provided by applicant):
PUF proteins are post-transcriptional repressors that control diverse processes such as development and differentiation, mitochondrial function, cellular growth, and lifespan. These sequence specific RNA-binding proteins bind to regulatory sequences in the 3' untranslated regions (3'UTRs) of target mRNAs to repress gene expression. The mechanism of repression by PUF proteins is not known. Our working hypothesis is that PUF proteins bind to the 3'UTR of an mRNA and recruit the basal mRNA turnover machinery to degrade that mRNA. We have chosen the yeast PUF5 protein as a model system for determining how PUF proteins function. Puf5p binds to and represses expression of the HO endonuclease mRNA, which is involved in mating type switching. We propose to identify protein-protein interactions between the Puf5p and yeast proteins using a two-hybrid screen. Also, biochemical purification of Puf5p protein complexes and protein microsequencing will be used to identify Puf5p-associated protein complexes. We will identify the components of the regulatory protein complex that assembles on the 3'UTR of the HO mRNA with Puf5p by using a three-hybrid protein-RNA interaction screen. An in vivo functional assay will be used to identify genes that are required for Puf5p-mediated repression and to identify the pathway of mRNA turnover that is controlled by Puf5p. Finally, an in vitro mRNA turnover assay will used to further dissect the mechanism of Puf5p repression.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Translational Control by Human Pumilio Proteins
-
批准号:10712307
-
项目类别:
-
资助金额:$37.97万
-
财政年份:2023
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
mRNA regulatory functions of the Drosophila TRIM-NHL protein, Brat
-
批准号:10794673
-
项目类别:
-
资助金额:$18.17万
-
财政年份:2022
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
mRNA regulatory functions of the Drosophila TRIM-NHL protein, Brat
-
批准号:10418852
-
项目类别:
-
资助金额:$29.44万
-
财政年份:2022
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
mRNA regulatory functions of the Drosophila TRIM-NHL protein, Brat
-
批准号:10670824
-
项目类别:
-
资助金额:$29.44万
-
财政年份:2022
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
Novel Regulatory Mechanisms of Drosophila Pumilio
-
批准号:10312121
-
项目类别:
-
资助金额:$32.12万
-
财政年份:2013
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
Novel regulatory mechanisms of Drosophila Pumilio and Nanos
-
批准号:8738690
-
项目类别:
-
资助金额:$28.67万
-
财政年份:2013
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
Novel regulatory mechanisms of Drosophila Pumilio and Nanos
-
批准号:8476706
-
项目类别:
-
资助金额:$28.69万
-
财政年份:2013
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
Request to Transfer R01GM105707-03 Novel regulatory mechanisms of Drosophila Pumilio and Nanos
-
批准号:9225476
-
项目类别:
-
资助金额:$25.86万
-
财政年份:2013
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
Request to Transfer R01GM105707-03 Novel regulatory mechanisms of Drosophila Pumilio and Nanos
-
批准号:9320859
-
项目类别:
-
资助金额:$28.85万
-
财政年份:2013
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
IDENTIFICATION OF PUF PROTEIN COMPLEXES
-
批准号:6979648
-
项目类别:
-
资助金额:$0.41万
-
财政年份:2004
-
负责人:Aaron Charles Goldstrohm
-
依托单位:
海外基金