Identification of Xcv TTSS components

描述（申请人提供）：植物致病菌油菜黄单胞菌pv。vesicatoria （Xcv）是番茄和辣椒细菌性斑疹病的病原体，其致病性需要一个功能良好的III型分泌系统（TTSS）。植物和动物的革兰氏阴性病原体都利用ttss将细菌效应蛋白直接引入宿主细胞质。TTSS装置是一个多组分复合物，由所有病原体中保守的蛋白质和每种病原体特有的蛋白质组成。关于11I型分泌所需的植物病原体特异性蛋白的身份或功能的信息很少。对这些蛋白的研究将有助于深入了解Xcv发病机制的初始阶段。

DESCRIPTION (provided by applicant): The plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) is the causative agent of bacterial spot disease in tomato and pepper, and requires a functional type III secretion system (TTSS) for its pathogenicity. Gram-negative pathogens of both plants and animals use TTSSs for introducing bacterial effector proteins directly into the host cell cytoplasm. The TTSS apparatus is a multi-component complex composed of proteins conserved in all pathogens and proteins unique to each pathogen. There is little information about the identity or function of plant pathogen-specific proteins required for type 11I secretion. The study of these proteins will provide insight into the initial stages of Xcv pathogenesis. The goal of the research in this proposal is to identify plant pathogen-specific components of the Xcv TTSS. Specifically, the TTSS complex will be biochemically isolated and the constituent proteins identified. Xcv-specific proteins will be characterized by determining their sub-cellular localization and their requirement for protein export from the bacteria and/or protein translocation through the host cell plasma membrane. The function of the Xcv HrpF protein, which is required for translocation of effectors into the plant cell, will also be investigated.