Identification of Xcv TTSS components

Xcv TTSS 组件的识别

• 批准号: 6876511
• 负责人: ALISON J DAVIS
• 金额: $ 5.15万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2006-04-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876511
• 关键词: Pseudomonadaceae; SDS polyacrylamide gel electrophoresis; bacteria infection mechanism; bacterial proteins; cell membrane; host organism interaction; mass spectrometry; membrane proteins; plant diseases; postdoctoral investigator; protein localization; protein protein interaction; protein purification; protein structure function; protein transport; secretory protein; western blottings

DESCRIPTION (provided by applicant): The plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) is the causative agent of bacterial spot disease in tomato and pepper, and requires a functional type III secretion system (TTSS) for its pathogenicity. Gram-negative pathogens of both plants and animals use TTSSs for introducing bacterial effector proteins directly into the host cell cytoplasm. The TTSS apparatus is a multi-component complex composed of proteins conserved in all pathogens and proteins unique to each pathogen. There is little information about the identity or function of plant pathogen-specific proteins required for type 11I secretion. The study of these proteins will provide insight into the initial stages of Xcv pathogenesis. The goal of the research in this proposal is to identify plant pathogen-specific components of the Xcv TTSS. Specifically, the TTSS complex will be biochemically isolated and the constituent proteins identified. Xcv-specific proteins will be characterized by determining their sub-cellular localization and their requirement for protein export from the bacteria and/or protein translocation through the host cell plasma membrane. The function of the Xcv HrpF protein, which is required for translocation of effectors into the plant cell, will also be investigated.

描述(申请人提供)：植物病原菌野油菜黄单胞菌。泡囊霉(vesicatoria，XCV)是引起番茄和辣椒细菌性斑点病的病原菌，其致病性需要一个功能性的III型分泌系统(TTSS)。植物和动物的革兰氏阴性病原体都使用TTSS将细菌效应蛋白直接引入宿主细胞细胞质。TTSS装置是由所有病原体中保守的蛋白质和每个病原体特有的蛋白质组成的多组分复合体。关于11I分泌所需的植物病原菌特异性蛋白的特性或功能的信息很少。对这些蛋白的研究将有助于深入了解XCV致病的初始阶段。 这项研究的目标是确定XCV TTSS的植物病原菌特异性成分。具体地说，将对TTSS复合体进行生化分离并鉴定其组成蛋白。XCV特异的蛋白质将通过确定它们的亚细胞定位和它们对从细菌中输出蛋白质和/或通过宿主细胞质膜的蛋白质转运的需求来表征。还将研究XCV HrpF蛋白的功能，这是效应器转移到植物细胞所必需的。