Identification of Xcv TTSS components

Xcv TTSS 组件的识别

• 批准号: 6784975
• 负责人: ALISON J DAVIS
• 金额: $ 2.84万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2006-04-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6784975
• 关键词: Pseudomonadaceae; SDS polyacrylamide gel electrophoresis; bacteria infection mechanism; bacterial proteins; cell membrane; host organism interaction; mass spectrometry; membrane proteins; plant diseases; postdoctoral investigator; protein localization; protein protein interaction; protein purification; protein structure function; protein transport; secretory protein; western blottings

DESCRIPTION (provided by applicant): The plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) is the causative agent of bacterial spot disease in tomato and pepper, and requires a functional type III secretion system (TTSS) for its pathogenicity. Gram-negative pathogens of both plants and animals use TTSSs for introducing bacterial effector proteins directly into the host cell cytoplasm. The TTSS apparatus is a multi-component complex composed of proteins conserved in all pathogens and proteins unique to each pathogen. There is little information about the identity or function of plant pathogen-specific proteins required for type 11I secretion. The study of these proteins will provide insight into the initial stages of Xcv pathogenesis. The goal of the research in this proposal is to identify plant pathogen-specific components of the Xcv TTSS. Specifically, the TTSS complex will be biochemically isolated and the constituent proteins identified. Xcv-specific proteins will be characterized by determining their sub-cellular localization and their requirement for protein export from the bacteria and/or protein translocation through the host cell plasma membrane. The function of the Xcv HrpF protein, which is required for translocation of effectors into the plant cell, will also be investigated.

描述（由申请人提供）：植物病原细菌野油菜黄单胞菌致病变种（Xanthomonascampestris pv.）水泡孢菌（Xcv）是番茄和辣椒细菌性斑点病的病原菌，其致病性需要功能性III型分泌系统（TTSS）。植物和动物的革兰氏阴性病原体使用TTSS将细菌效应蛋白直接引入宿主细胞质中。TTSS装置是由所有病原体中保守的蛋白质和每种病原体特有的蛋白质组成的多组分复合物。关于11型I分泌所需的植物病原体特异性蛋白的身份或功能的信息很少。这些蛋白质的研究将提供深入了解Xcv发病机制的初始阶段。 本研究的目的是确定Xcv TTSS的植物病原体特异性成分。具体而言，TTSS复合物将被生物化学分离并鉴定组成蛋白。Xcv特异性蛋白质将通过确定其亚细胞定位和其从细菌输出蛋白质和/或通过宿主细胞质膜转运蛋白质的需求来表征。还将研究Xcv HrpF蛋白的功能，该蛋白是将效应子易位到植物细胞中所需的。