lin 4 independent translational repression of lin 28

lin 4 对 lin 28 的独立翻译抑制

• 批准号: 6698080
• 负责人: Allison Lynn Abbott
• 金额: $ 4.89万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6698080
• 关键词: Caenorhabditis elegans; developmental genetics; gene induction /repression; gene mutation; genetic translation; genetically modified animals; green fluorescent proteins; hormone receptor; larva; messenger RNA; reporter genes; transcription factor

DESCRIPTION (provided by applicant) In C. elegans post-embryonic developmental timing is regulated by heterochronic genes. The temporal downregulation of lin-28 is necessary for the execution of the larval stage 3 (L3) program, a novel pathway independent of lin-4 involving the nuclear hormone receptor daf-12 acts to repress lin-28. The aims of this proposal are to determine the molecular mechanism of lin-4-independent repression (LIR) of lin-28 and to identify genes essential for this timing mechanism. First, the hypothesis will be tested that daf-12 translationally represses lin-28 and thereby specifies entry into the L3 stage. To test this, I will analyze lin-28 mRNA and protein levels during larval development in wildtype and daf-12 mutant genotypes. Secondly, the hypothesis will be tested that elements in the 3' UTR of lin-28 are sufficient for LIR-dependent downregulation of lin-28 and are required for entry into L3. GFP reporter constructs with intact or truncated lin-28 3' UTR sequences will be used to test sufficiency and identify specific LIR elements. Lastly, a genetic screen will be performed to identify new components of the LIR pathway. This proposal has the exciting potential to identify new small temporal RNAs regulators of developmental timing, which is emerging as a common mode of regulation from mouse to man.

描述（由申请人提供）