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Functional Analysis of microRNAs in C. elegans spermatogenesis

微小RNA在秀丽隐杆线虫精子发生中的功能分析

基本信息

批准号：
9442465
负责人：
Allison Lynn Abbott
金额：
$ 44.98万
依托单位：
MARQUETTE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-19 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9442465
关键词：
Address Animals Basic Science Biogenesis Caenorhabditis elegans Collaborations Critical Thinking Data Analyses Defect Development Diagnostic Education Etiology Event Family Family member Fertility Fertility Disorders Fluorescence Microscopy Future Gene Expression Gene Targeting Genetic Germ Cells Germ Lines Goals Gonadal structure In Situ Hybridization Individual Infertility Investigation Male Infertility Malignant neoplasm of testis MicroRNAs Molecular Oocytes Oral Organism Pathway interactions Patients Phenotype Physiology Process Production Publications RNA Interference Regulation Regulator Genes Research Resources Role Scientist Spermatogenesis Students System Techniques Testicular Germ Cell Tumor Tissues Training Work design experimental study genetic approach gonad function graduate student insight knock-down male meetings mutant next generation sequencing skills sperm cell sperm function student mentoring treatment strategy undergraduate student

项目摘要

7. PROJECT SUMMARY The production of functional gametes is of central importance to the survival of all sexually reproducing species. Our goal is to identify the microRNAs that function to control the essential process of spermatogenesis in C. elegans. microRNAs are indispensable regulators of gene expression that are required for animal development and physiology. microRNAs have been found to be misregulated in patients with male factor infertility as well as in testicular germ cell tumors. However, the specific pathways and targets regulated by individual microRNAs in the process of spermatogenesis remain largely unknown. The study of microRNAs in C. elegans has been instrumental in defining the core principles of individual microRNA regulation of targets in developmental pathways. This work will provide new insights into the principles of microRNA function in regulatory networks that function in the process of spermatogenesis. microRNAs are required for mammalian spermatogenesis but their role in C. elegans spermatogenesis remains uncharacterized. This work will address three central questions: 1) What specific events of spermatogenesis require microRNAs? 2) What individual microRNAs function are expressed in the male gonad and function to regulate spermatogenesis? and 3) What pathways and direct targets are regulated the mir-44 family of microRNAs? This work will identify specific defects in spermatogenesis, pathways, and direct targets regulated by the mir-44 family. This work will provide a resource for future investigation to comprehensively define the functions of the subset of microRNAs that act in the male germ line to regulate the process of spermatogenesis.

7.项目摘要功能性配子的产生对所有有性生殖的生物的生存至关重要物种我们的目标是确定微RNA的功能，以控制的基本过程，精子发生在C.优美的microRNA是基因表达不可或缺的调节因子，动物的发育和生理学。已经发现microRNA在男性乳腺癌患者中被错误调节，不育因素以及睾丸生殖细胞肿瘤。然而，调控的特定途径和靶点在精子发生过程中个体microRNA的作用仍然是未知的。microRNA的研究 in C.线虫在定义个体microRNA调节靶点的核心原则方面发挥了重要作用在发展的道路上。这项工作将提供新的见解microRNA功能的原则，在精子发生过程中起作用的调节网络。 microRNA是哺乳动物精子发生所必需的，但它们在C.秀丽隐翅虫精子发生仍然没有特征。这项工作将解决三个中心问题：1）什么具体的事件，精子发生需要microRNA？2)男性生殖腺中表达的单个microRNA功能是什么和调节精子生成的功能mir-44调控的途径和直接靶点是什么 microRNA家族？这项工作将确定在精子发生，途径和直接目标的具体缺陷由mir-44家族控制这项工作将为今后的研究提供一个资源，定义在男性生殖细胞系中起作用的microRNA子集的功能，以调节精子发生