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Genetic Analysis of Conserved microRNAs in C. elegans

线虫中保守 microRNA 的遗传分析

基本信息

批准号：
8689310
负责人：
Allison Lynn Abbott
金额：
$ 34.31万
依托单位：
MARQUETTE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-04-01 至 2017-09-18
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8689310
关键词：
Accounting Address Adult Animals Behavior Biogenesis Biological Biological Models Biological Process Caenorhabditis elegans Calcium Calcium Oscillations Cardiovascular Diseases Communication Complex Critical Thinking Data Data Analyses Defecation Defect Development Developmental Process Diabetes Mellitus Education Eph Family Receptors Epidermal Growth Factor Event Fertility Foundations Gene Expression Genes Genetic Germ Cells Goals Gonadal structure Health High-Throughput Nucleotide Sequencing Human In Situ Hybridization Individual Inositol Intestines Life Longevity Malignant Neoplasms Mammals Mediating Messenger RNA MicroRNAs Molecular Analysis Molecular Genetics Mutation Neurodegenerative Disorders Oral Organism Ovulation Pathway interactions Phenotype Physiological Physiological Processes Physiology Process Proteins Publications RNA Sequences Receptor Protein-Tyrosine Kinases Regulation Regulator Genes Regulatory Pathway Reporter Research Role Scientist Staging Sterility Students System Techniques Temperature Time Tissues Training Transgenes Work Writing design fly genetic analysis gonad function human disease loss of function meetings mutant neuronal cell body public health relevance recombinase reproductive research study skills temperature sensitive mutant undergraduate student

项目摘要

DESCRIPTION (provided by applicant): The long term goal of this work is to define how microRNAs fit into complex developmental and physiological networks. The identification of pathways and targets regulated by individual microRNAs is vital to understand microRNA function in animal development and in human disease. As an eminently genetically-tractable animal, C. elegans provides an ideal model system in which to study the functions of microRNAs, particularly as many microRNAs show complete or near-complete conservation between worms and humans. The proposed research is focused on the single biological process of ovulation. Ovulation is a calcium-dependent rhythmic behavior that requires microRNAs. The central questions of this work are: 1) what specific events of ovulation require microRNAs? 2) What individual microRNAs function in the germ cells or somatic gonad to regulate ovulation and 3) what pathways and direct targets are regulated by these microRNAs? To address these questions we will use genetic approaches to conditionally reduce microRNA biogenesis activity to determine specific ovulation events that require microRNAs. In addition, we will use high-throughput sequencing to profile microRNAs that are expressed in the somatic gonad. Lastly, we will identify which somatic gonad microRNAs contribute to the control of ovulation and determine the pathways and targets that they regulate. This comprehensive analysis of a single biological process provides an opportunity to define how microRNA's act in complex regulatory networks.

描述(由申请人提供)：这项工作的长期目标是定义microRNAs如何适应复杂的发育和生理网络。识别由单个microRNAs调控的途径和靶点对于了解microRNA在动物发育和人类疾病中的功能至关重要。线虫作为一种遗传易感性的动物，为研究microRNAs的功能提供了一个理想的模型系统，特别是因为许多microRNAs在蠕虫和人类之间显示出完全或接近完全的保守性。建议的研究集中在排卵的单一生物过程。排卵是一种钙依赖的节律行为，需要microRNA。这项工作的中心问题是：1)排卵的哪些特定事件需要microRNAs？2)生殖细胞或体细胞性腺中有哪些单独的microRNAs调节排卵，以及3)这些microRNAs调控哪些途径和直接靶点？为了解决这些问题，我们将使用遗传方法来有条件地降低microRNA的生物发生活性，以确定需要microRNA的特定排卵事件。此外，我们将使用高通量测序来分析在体细胞性腺中表达的microRNA。最后，我们将确定哪些体细胞性腺微RNA有助于控制排卵，并确定它们调节的途径和靶点。这种对单个生物过程的全面分析提供了一个机会来定义microRNA如何在复杂的调控网络中发挥作用。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Functional analysis of microRNA pathway genes in the somatic gonad and germ cells during ovulation in C. elegans.

线虫排卵期间体细胞性腺和生殖细胞中 microRNA 途径基因的功能分析。

DOI：
10.1016/j.ydbio.2017.04.007
发表时间：
2017
期刊：
Developmental biology
影响因子：
2.7
作者：
Rios,Carmela;Warren,David;Olson,Benjamin;Abbott,AllisonL
通讯作者：
Abbott,AllisonL

Stage-Specific Timing of the microRNA Regulation of lin-28 by the Heterochronic Gene lin-14 in Caenorhabditis elegans.

秀丽隐杆线虫中异时基因 lin-14 对 lin-28 的 microRNA 调节的阶段特异性时序。

DOI：
10.1534/genetics.116.195040
发表时间：
2017
期刊：
Genetics
影响因子：
3.3
作者：
Tsialikas,Jennifer;Romens,MitchellA;Abbott,Allison;Moss,EricG
通讯作者：
Moss,EricG

Loss of individual microRNAs causes mutant phenotypes in sensitized genetic backgrounds in C. elegans.

DOI：
10.1016/j.cub.2010.05.062
发表时间：
2010-07-27
期刊：
CURRENT BIOLOGY
影响因子：
9.2
作者：
Brenner, John L.;Jasiewicz, Kristen L.;Fahley, Alisha F.;Kemp, Benedict J.;Abbott, Allison L.
通讯作者：
Abbott, Allison L.

The mir-51 family of microRNAs functions in diverse regulatory pathways in Caenorhabditis elegans.