The effects of strain on vocal fold fibroblasts

描述(申请人提供)：目前的资金申请提出了一系列实验，这些实验将定量描述细胞对炎症的反应，并进一步尝试在体外模型中量化循环拉伸应变的影响。培养的声带成纤维细胞将暴露于IL-1β，这种方法已被证明在体外可以在不同类型的细胞中诱导无数的促炎作用，包括刺激环氧合酶-2(COX-2)和一氧化氮(NO)，以及抑制蛋白多糖的产生。然后，成纤维细胞将受到循环拉伸应变(CTS)，模拟(大致)体外声带振荡。据推测，CTS将作为许多IL-1β依赖的促炎作用的拮抗剂。这一发现在许多其他类型的细胞中得到了证实。目前的研究希望提供数据，表明声带振荡作为一种有效的抗炎剂的作用，以及具有长期的组织重塑意义。此外，它还试图结合赞助商目前的NIH投资回报率提供体外数据。

DESCRIPTION (provided by applicant): The current request for funding proposes a series of experiments which will quantitatively describe the cellular response to inflammation and, further, attempt to quantify the effects of cyclic tensile strain in an in vitro model. Fibroblasts cultured from the vocal folds will be exposed to IL-1beta, a method which has been shown to elicit a myriad of pro-inflammatory actions in various cell types in vitro including the stimulation of cyclo-oxygenase-2 (COX-2) and nitric oxide (NO), as well as the inhibition of proteoglycan production. Fibroblasts will then be subjected to cyclic tensile strain (CTS), simulating (roughly) in vitro vocal fold oscillation. It is hypothesized that CTS will act as an antagonist to many of the IL-1beta-dependent proinflammatory actions. This finding has been confirmed in numerous other cell types. The current investigation hopes to provide data suggesting a role for vocal fold oscillations as a potent anti-inflammatory agent as well as having long-term tissue remodeling implications. Furthermore, it seeks to provide in vitro data in conjunction with the Sponsor's current NIH ROI.