The effects of strain on vocal fold fibroblasts

描述（由申请人提供）：目前的资金申请提出了一系列实验，这些实验将定量描述细胞对炎症的反应，并进一步尝试量化体外模型中循环拉伸应变的影响。将从声带培养的成纤维细胞暴露于IL-1 β，IL-1 β是一种已显示在体外引起各种细胞类型中的无数促炎作用的方法，包括刺激环加氧酶-2（考克斯-2）和一氧化氮（NO），以及抑制蛋白聚糖产生。然后，成纤维细胞将经受循环拉伸应变（CTS），模拟（粗略地）体外声带振荡。假设CTS将作为许多IL-1 β依赖性促炎作用的拮抗剂。这一发现已在许多其他细胞类型中得到证实。目前的研究希望提供的数据表明，声带振荡作为一种有效的抗炎剂的作用，以及具有长期的组织重塑的影响。此外，其旨在结合申办方当前的NIH ROI提供体外数据。

DESCRIPTION (provided by applicant): The current request for funding proposes a series of experiments which will quantitatively describe the cellular response to inflammation and, further, attempt to quantify the effects of cyclic tensile strain in an in vitro model. Fibroblasts cultured from the vocal folds will be exposed to IL-1beta, a method which has been shown to elicit a myriad of pro-inflammatory actions in various cell types in vitro including the stimulation of cyclo-oxygenase-2 (COX-2) and nitric oxide (NO), as well as the inhibition of proteoglycan production. Fibroblasts will then be subjected to cyclic tensile strain (CTS), simulating (roughly) in vitro vocal fold oscillation. It is hypothesized that CTS will act as an antagonist to many of the IL-1beta-dependent proinflammatory actions. This finding has been confirmed in numerous other cell types. The current investigation hopes to provide data suggesting a role for vocal fold oscillations as a potent anti-inflammatory agent as well as having long-term tissue remodeling implications. Furthermore, it seeks to provide in vitro data in conjunction with the Sponsor's current NIH ROI.