5-HT1A receptor and hallucinogens

5-HT1A受体和致幻剂

• 批准号: 6804047
• 负责人: Chad J. Reissig
• 金额: $ 2.87万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-12 至 2006-09-11
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6804047
• 关键词: 2,5 dimethoxy 4 methylamphetamine; G protein; chemical stimulation; glutamates; guanine nucleotide binding protein; hallucinogens; high performance liquid chromatography; indoleamine; laboratory rat; ligands; microdialysis; pharmacokinetics; phenylethylamines; pons; predoctoral investigator; protein structure function; psychopharmacology; receptor binding; serotonin; serotonin receptor; stimulus /response

DESCRIPTION (provided by applicant): The use of hallucinogens in our society, particularly adolescents and young adults, continues to be widespread and is thus a major interest of the National Institute on Drug Abuse. The aim of the proposed project is to elucidate the modulatory role of serotonergic receptors of the 5-HTIA subtype in the actions of hallucinogens. This will be accomplished using behavioral and biochemical techniques to determine [a] the modulatory effects of 5-HT1A receptor agonists on stimulus control by DOM, a phenethylamine hallucinogen, [b] the specific role of 5-HT1A receptors of the dorsal and medial raphe nuclei in DOM induced stimulus control, [c] the role of G protein activation in the interactions of 5-HTIA agonists with DOM, and [d] possible serotonergic/glutamatergic interactions as they relate to 5-HT1A ligands. The behavioral variable in these studies will be DOM-induced stimulus control. Glutamate levels will be determined by in vivo microdialysis and HPLC. [35S] GTPgammaS binding will be used to identify molecular mechanisms involved in 5-HT1A receptor stimulation.

描述（由申请人提供）： 致幻剂的使用在我们的社会中，特别是青少年和年轻人中，仍然很普遍，因此是国家药物滥用研究所的主要兴趣。该项目的目的是阐明 5-HTIA 亚型血清素受体在致幻剂作用中的调节作用。这将使用行为和生化技术来完成，以确定 [a] 5-HT1A 受体激动剂对 DOM（一种苯乙胺致幻剂）刺激控制的调节作用，[b] 中缝背核和中缝核的 5-HT1A 受体在 DOM 诱导的刺激控制中的具体作用，[c] G 蛋白激活在 5-HTIA 激动剂与 DOM 相互作用中的作用 DOM，和[d]可能的血清素能/谷氨酸能相互作用，因为它们与 5-HT1A 配体有关。这些研究中的行为变量将是 DOM 诱导的刺激控制。谷氨酸水平将通过体内微透析和HPLC测定。 [35S] GTPgammaS 结合将用于鉴定参与 5-HT1A 受体刺激的分子机制。