Does Tel2p act DNA double-strand breaks?

项目描述（申请人提供）：本项目总体目标是以酿酒酵母菌为模型系统，运用生物信息学和遗传学方法研究其寿命控制。具体来说，本研究将使用多个长寿命和短寿命突变体的转录谱来鉴定有丝分裂和有丝分裂后衰老所需的基因。所鉴定的基因将根据与改变寿命相关的表型以及它们对正常寿命的要求分组为遗传途径。寿命控制途径的调控成分将使用生物信息学工具来确定统计上显着的过度代表的功能相关基因组和可能调节其转录的序列元件。这些研究结果将为理解所有模式生物的寿命控制机制提供一个有价值的模型系统。

DESCRIPTION (provided by applicant): The overall goal of this project is to apply bioinformatic and genetic approaches to study the control of life span using Saccharomyces cerevisiae as a model system. Specifically, this study will use transcriptional profiling of multiple long-lived and short-lived mutants to identify genes required for mitotic and post-mitotic aging. The genes identified will be grouped into genetic pathways based on phenotypes associated with altered life span as well as their requirement for normal life span. Regulatory components of the life span controlling pathways will be identified using bioinformatics tools to determine statistically significant over-represented groups of functionally related genes and sequence elements that may regulate their transcription. The results from these studies will provide a valuable model system for understanding the mechanism of life span control in all model organisms.