Does Tel2p act DNA double-strand breaks?

描述（由申请人提供）：本项目的总体目标是应用生物信息学和遗传学方法，以酿酒酵母为模型系统研究寿命控制。具体而言，本研究将使用多个长寿和短命突变体的转录谱，以确定有丝分裂和有丝分裂后衰老所需的基因。根据与寿命改变相关的表型及其对正常寿命的要求，将确定的基因分为遗传途径。将使用生物信息学工具来确定寿命控制途径的调节组分，以确定可能调节其转录的功能相关基因和序列元件的统计学显著的过度代表组。这些研究结果将为理解所有模式生物的寿命控制机制提供一个有价值的模型系统。

DESCRIPTION (provided by applicant): The overall goal of this project is to apply bioinformatic and genetic approaches to study the control of life span using Saccharomyces cerevisiae as a model system. Specifically, this study will use transcriptional profiling of multiple long-lived and short-lived mutants to identify genes required for mitotic and post-mitotic aging. The genes identified will be grouped into genetic pathways based on phenotypes associated with altered life span as well as their requirement for normal life span. Regulatory components of the life span controlling pathways will be identified using bioinformatics tools to determine statistically significant over-represented groups of functionally related genes and sequence elements that may regulate their transcription. The results from these studies will provide a valuable model system for understanding the mechanism of life span control in all model organisms.