Plasticity of Spinal Inhibition in Spinal Cord Injury
脊髓损伤中脊髓抑制的可塑性
基本信息
- 批准号:6836863
- 负责人:
- 金额:$ 2.54万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-08-09 至 2006-08-08
- 项目状态:已结题
- 来源:
- 关键词:animal tissuecell population studyelectrophysiologygamma aminobutyrategene expressiongene expression profilinggenetic regulationgenetically modified animalsinterneuronslaboratory mouselaser capture microdissectionmicroarray technologynervous system regenerationneural information processingneural inhibitionneural plasticityneurochemistryneurogeneticsneuroregulationpainpredoctoral investigatorprotein structure functionsomesthetic sensory cortexspinal cord injuryvoltage gated channel
项目摘要
DESCRIPTION (provided by applicant):
GABAergic neurons can be identified in transgenic mice expressing GFP under the control of a GAD67 regulatory element (Oliva et al., 2000). Whole-cell patch recordings permit characterization of the intrinsic membrane properties of visually-identified GABAergic neurons. To study the modifiability of these neurons, their intrinsic and monoaminergic modulatory properties will be characterized in mice with and without chronic spinal transection. Parallel DNA expression profiling of the same population of neurons with LCM will be performed to identify transection-induced alterations in gene expression. Together, these studies will identify and inter-relate physiologic and molecular plasticity in identified GABAergic interneurons. The long-term goal is to identify fundamental molecular and physiological circuits engaged in the control of the spinal inhibitory apparatus. Clinically, identification of the specific monoaminergic receptor subtypes on GABAergic neurons in the spinal cord and the way in which their firing properties are modulated should identify targets for selective control of GABAergic excitability. This can then be manipulated to limit enhanced sensory transmission in lamina I that is thought to occur in various pain states and after SCI.
描述(由申请人提供):
可以在GAD 67调节元件控制下表达GFP的转基因小鼠中鉴定GABA能神经元(奥利瓦(Oliva)等人,2000)。全细胞斑片记录允许表征视觉识别的GABA能神经元的内在膜特性。为了研究这些神经元的可修饰性,将在有和没有慢性脊髓横断的小鼠中表征其内在和单胺能调节特性。将使用LCM对相同神经元群体进行平行DNA表达谱分析,以鉴定横切诱导的基因表达变化。总之,这些研究将确定GABA能中间神经元的生理和分子可塑性并将其相互关联。长期目标是确定参与脊髓抑制装置控制的基本分子和生理回路。在临床上,确定脊髓中GABA能神经元上的特定单胺能受体亚型以及它们的放电特性被调制的方式,应该确定用于选择性控制GABA能兴奋性的靶点。然后可以操纵这一点来限制被认为在各种疼痛状态和SCI后发生的椎板I中的增强的感觉传递。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Kimberly J Dougherty其他文献
Kimberly J Dougherty的其他文献
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{{ truncateString('Kimberly J Dougherty', 18)}}的其他基金
Mechanisms of locomotor rhythm generation in rodent spinal cord
啮齿动物脊髓运动节律的产生机制
- 批准号:
10708988 - 财政年份:2022
- 资助金额:
$ 2.54万 - 项目类别:
Mechanisms of locomotor rhythm generation in rodent spinal cord
啮齿动物脊髓运动节律的产生机制
- 批准号:
10605444 - 财政年份:2022
- 资助金额:
$ 2.54万 - 项目类别:
Specific spinal locomotor circuit alterations induced by epidural stimulation
硬膜外刺激引起的特定脊髓运动回路改变
- 批准号:
10041067 - 财政年份:2020
- 资助金额:
$ 2.54万 - 项目类别:
Crucial spinal circuit changes that mediate locomotion benefits of combined biological/bionic/rehabilitation therapies after spinal cord injury.
脊髓损伤后联合生物/仿生/康复治疗的关键脊髓回路变化可调节运动益处。
- 批准号:
10213148 - 财政年份:2018
- 资助金额:
$ 2.54万 - 项目类别:
Crucial spinal circuit changes that mediate locomotion benefits of combined biological/bionic/rehabilitation therapies after spinal cord injury.
脊髓损伤后联合生物/仿生/康复治疗的关键脊髓回路变化可调节运动益处。
- 批准号:
10447027 - 财政年份:2018
- 资助金额:
$ 2.54万 - 项目类别:
CRCNS: Rhythm generation in rodent spinal cord
CRCNS:啮齿动物脊髓节律的产生
- 批准号:
9114688 - 财政年份:2015
- 资助金额:
$ 2.54万 - 项目类别:
CRCNS: Rhythm generation in rodent spinal cord
CRCNS:啮齿动物脊髓节律的产生
- 批准号:
9325618 - 财政年份:2015
- 资助金额:
$ 2.54万 - 项目类别:
Plasticity of Spinal Inhibition in Spinal Cord Injury
脊髓损伤中脊髓抑制的可塑性
- 批准号:
6938536 - 财政年份:2004
- 资助金额:
$ 2.54万 - 项目类别: