Turnover of Musculotendinous Collagen Following Exercise

运动后肌腱胶原蛋白的周转

• 批准号: 6880300
• 负责人: Benjamin Francis Miller
• 金额: $ 0.28万
• 依托单位: BISPEBJERG HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6880300
• 关键词: Scandinavian country; clinical research; collagen; connective tissue development; connective tissue metabolism; exercise; human subject; knee; male; microdialysis; muscle metabolism; musculoskeletal system; postdoctoral investigator; protein metabolism; radiotracer; repetitive motion injury; sports injury; tendon injury; tendons; young adult human (21-34)

DESCRIPTION (provided by applicant): Overuse injuries are common to those regularly performing activity as well as those with job related injuries. The site of failure in chronic overuse injuries are often musculotendinous structures. The most abundant component of musculotendinous tissue is collagen. Despite its ubiquity, collagen tissue is not well understood and is perceived to be rather inert. However, evidence is accumulating that collagen is quite dynamic. The purpose of this proposal is to further elucidate the processes of muscle and tendon collagen synthesis and degradation in response to mechanical loading and its deficiencies in overuse injuries. Specifically it is hypothesized that, 1) muscle and tendon collagen increase synthesis in response to exercise but these rates do not follow the same time course, 2) rates of muscle and tendon collagen degradation increase in response to exercise, 3) in subjects with an overuse tendon injury, rates of collagen synthesis in tendon (but not muscle) are transiently decreased compared to healthy tendon after an acute bout of exercise. To this end muscle collagen synthesis and degradation and its regulation will be studied with a combination of stable isotope tracers, tissue sampling, microdialysis, and molecular biology techniques during recovery from a strenuous bout of exercise in healthy subjects and those with patellar tendonitis. With elucidation of the patterns of collagen synthesis and degradation and where deficiencies lie in those with injuries, it will then be possible to design specific interventions to aid in restructuring.

描述（由申请人提供）：过度使用伤害对于那些经常进行活动以及因工作受伤的人来说很常见。慢性过度使用损伤的失效部位通常是肌肉肌腱结构。肌腱组织最丰富的成分是胶原蛋白。尽管胶原组织无处不在，但人们对它的了解还不是很多，并且被认为是相当惰性的。然而，越来越多的证据表明胶原蛋白是非常活跃的。该提案的目的是进一步阐明肌肉和肌腱胶原蛋白合成和降解的过程，以响应机械负荷及其在过度使用损伤中的缺陷。具体来说，假设：1）肌肉和肌腱胶原蛋白的合成因运动而增加，但这些速率并不遵循相同的时间进程，2）肌肉和肌腱胶原蛋白的降解速率因运动而增加，3）在过度使用肌腱损伤的受试者中，与健康肌腱相比，在剧烈运动后，肌腱（而非肌肉）中胶原蛋白的合成速率短暂降低。为此，将结合稳定同位素示踪剂、组织取样、微透析和分子生物学技术，在健康受试者和患有髌腱炎的受试者从剧烈运动恢复期间研究肌肉胶原蛋白的合成和降解及其调节。通过阐明胶原蛋白合成和降解的模式以及受伤者的缺陷所在，就可以设计具体的干预措施来帮助重建。