Development of a biocatalytic route for the sustainable synthesis of amino-alcohols and amino sugars in industry

目标：这个博士项目将专注于应用已建立的高通量筛选(HTS)来有效地设计一种新型的嗜盐转氨酶HE-AT，以增强其对广泛的单糖的活性并优化其稳定性(热稳定性、溶剂性和pH稳定性)。本研究的总体目标是使单糖生物催化转化为氨基醇/氨基糖成为这些分子的中小型和工业化合成的“首选”方法。具体的项目里程碑/目标是：(1)针对商用的醛糖/酮糖筛选野生型TA(2)使用我们的HT筛选方法(方案1)将生物催化剂进化为单糖(3)使用测序数据微调生物催化(4)筛选德国巴斯夫AT生物催化的优化小组，以评估这些催化剂在真实工业条件下的适用性。“

"Aims: This PhD project will focus on the application of an established high-throughput screen (HTS) for the efficient engineering of a novel halophilic transaminase, HE-AT in order to enhance its activity towards a broad range of simple sugars and optimise its stability (thermos-, solvent- and pH-stability). The overall aim of this reseach is to make the biocatalytic conversion of simple sugars to amino alcohols/amino sugars the 'go to' method for small/medium and industrial scale synthesis of these molecules. The specific project milestones/objectives are:(1) Screen the wild-type TA against commercially available aldoses/ketoses(2) Employ our HT screening methodology (scheme 1) to evolve the biocatalyst towards simple sugars(3) Use sequencing data to fine-tune the biocatalysis(4) Screen the optimised panel of AT biocatalysis at BASF in Germany to assess the suitability of these catalysts under authentic industrial conditions."