Development of a biocatalytic route for the sustainable synthesis of amino-alcohols and amino sugars in industry

开发用于工业可持续合成氨基醇和氨基糖的生物催化路线

• 批准号: 2422051
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2422051
• 关键词: Development; biocatalytic; route; sustainable; synthesis

"Aims: This PhD project will focus on the application of an established high-throughput screen (HTS) for the efficient engineering of a novel halophilic transaminase, HE-AT in order to enhance its activity towards a broad range of simple sugars and optimise its stability (thermos-, solvent- and pH-stability). The overall aim of this reseach is to make the biocatalytic conversion of simple sugars to amino alcohols/amino sugars the 'go to' method for small/medium and industrial scale synthesis of these molecules. The specific project milestones/objectives are:(1) Screen the wild-type TA against commercially available aldoses/ketoses(2) Employ our HT screening methodology (scheme 1) to evolve the biocatalyst towards simple sugars(3) Use sequencing data to fine-tune the biocatalysis(4) Screen the optimised panel of AT biocatalysis at BASF in Germany to assess the suitability of these catalysts under authentic industrial conditions."

“目的：该博士项目将重点关注已建立的高通量筛选（HTS）的应用，用于新型嗜盐转氨酶HE-AT的高效工程设计，以增强其对多种单糖的活性并优化其稳定性（热稳定性，溶剂稳定性和ph稳定性）。本研究的总体目标是使单糖转化为氨基醇/氨基糖的生物催化转化成为中小规模和工业规模合成这些分子的“必经”方法。具体的项目里程碑/目标是：(1)根据市上可用的醛糖/酮糖筛选野生型TA(2)采用我们的HT筛选方法（方案1）将生物催化剂向单糖方向发展(3)使用测序数据微调生物催化(4)在德国巴斯夫筛选优化的AT生物催化板，以评估这些催化剂在真实工业条件下的适用性。”