Real time sequencing for assembly of complex genomic regions using selective sequencing

使用选择性测序对复杂基因组区域进行实时测序

• 批准号: 2422574
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2422574
• 关键词: Real; time; sequencing; assembly; complex

My research interests are focused upon the application of computational and genetic techniques to problems in the life sciences. I have found population genetics and evolutionary-developmental biology to be particularly fascinating as they combine understanding how genes interact to create life and, how genetic differences lead to diversity. To further explore these areas I undertook a dissertation project, supervised by Professor Alistair McGregor, where I investigated the spatiotemporal expression of paralogous genes in the spider Parasteatoda tepidariorum; this project primarily used molecular techniques to clone and generate probes for genes of interest that were used in hybridisation experiments. Furthermore, I am also pursuing an independent study with Dr Daniela Santos-Nunes to identify polymorphic sites in Drosophila simulans and D. mauritiana using population genomic data obtained from pooled sequencing and to develop a web application that allows nucleotide by nucleotide comparison for both species. I am an organised and motivated student. The research that I have undertaken, as part of my undergraduate course, has provided me with a range of skills in both molecular biology and bioinformatics; and, through attending weekly research seminars and discussions with researchers from across the UK. I have gained insight into how research progresses in academia. Moreover, my position with the IT Services department has developed my general technical knowledge and problem solving skills, while allowing me to gain experience writing technical documentation.

我的研究兴趣集中在计算和遗传技术在生命科学问题中的应用。我发现种群遗传学和进化发育生物学特别吸引人，因为它们结合了对基因如何相互作用创造生命以及基因差异如何导致多样性的理解。为了进一步探索这些领域，我在Alistair McGregor教授的指导下进行了一个论文项目，在那里我研究了拟龙蛛（Parasteatoda tepidariorum）中副同源基因的时空表达；该项目主要使用分子技术克隆和生成用于杂交实验的感兴趣基因探针。此外，我还在与Daniela Santos-Nunes博士进行一项独立研究，利用从汇总测序中获得的种群基因组数据，确定拟果蝇和毛里求斯果蝇的多态性位点，并开发一个网络应用程序，允许对这两个物种进行核苷酸比对。我是一个有组织有上进心的学生。作为本科课程的一部分，我所从事的研究为我提供了分子生物学和生物信息学方面的一系列技能；每周参加研究研讨会，与来自英国各地的研究人员进行讨论。我对学术界的研究进展有了更深入的了解。此外，我在IT服务部的职位发展了我的一般技术知识和解决问题的能力，同时也使我获得了编写技术文档的经验。