Animal Resources and Pathology Core

动物资源和病理学核心

• 批准号: 6990347
• 负责人: JAMES G FOX
• 金额: $ 14.89万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-13 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990347
• 关键词: animal colony; biomedical facility; histopathology; laboratory mouse

This Core is essential for the delivery of optimal veterinary care and facilities utilized for the conduct of in vivo experimentation. The PI.'s of this program project will have access to the Division of Comparative Medicine's AAALAC accredited, centrally managed and operated 125,000 sq. ft. of space, located in five buildings at the Institute. Each of the units has been built or extensively renovated in the last eight years. Four of these buildings have three corridor systems and three have specialized bio-containment units for infectious or hazardous chemical experimentation. Barrier facilities for housing specific pathogen free transgenic mouse colonies are also available. These animal facilities provide MIT investigators a controlled environment with the necessary space, equipment, and containment facilities for performing biomedical related research in animals including germ-flee work and also ensures personnel safety during the experimental process. The DCM Comparative Pathology Laboratory will also provide the personnel and expertise to perform histological and immunocytochemical analysis of animal tissues delivered from in vivo studies performed within the framework of the program project.Specifically the role of Core 2 is to provide facilities, equipment, and personnel with expertise to centrally manage and supervise animal husbandry and experimental animal manipulations and provide histopathologic and transgenic services involved in studies conducted in this Program Project Grant. The specific aims are as follows: Specific aim #1. To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen free status of GEM used in this program Specific aim #2. Provide colony management and disease prevention of GEM for use by program project PI's and as needed generate transgenic animals by pronuclear microinjection of DNA constructs or embryonic stem cell manipulation Specific aim #3. To provide histology, pathology and molecular characterization expertise for extensive phenotyping and assessment of pathological damage of mouse models used in this program.

该核心对于提供最佳兽医护理和用于进行体内实验的设施至关重要。私家侦探。该计划项目的参与者将可以使用比较医学部的AAALAC认证，集中管理和运营的125，000平方米。英尺空间，位于研究所的五座建筑物中。每一个单位都是在过去八年中建造或大规模翻修的。其中四座建筑物有三个走廊系统，三座建筑物有专门的生物封闭单元，用于进行传染性或危险化学品实验。还提供了用于安置无特定病原体转基因小鼠群体的屏障设施。这些动物设施为麻省理工学院的研究人员提供了一个受控的环境，具有必要的空间，设备和收容设施，用于在动物中进行生物医学相关的研究，包括细菌逃逸工作，并确保实验过程中的人员安全。DCM比较病理学实验室还将提供人员和专业知识，对在该项目框架内进行的体内研究提供的动物组织进行组织学和免疫细胞化学分析。具体而言，核心2的作用是提供设施、设备、和专业人员，集中管理和监督畜牧业和实验动物操作，并提供组织病理学和转基因参与本计划项目补助金研究的服务。具体目标如下： 具体目标#1。通过胚胎移植重新获得转基因和其他特定小鼠品系，以确保本项目中使用的GEM无特定病原体状态 具体目标#2提供GEM的菌落管理和疾病预防，供项目PI使用，并根据需要通过DNA构建体的原核显微注射或胚胎干细胞操作产生转基因动物 具体目标#3提供组织学、病理学和分子表征专业知识，用于本项目中使用的小鼠模型的广泛表型分析和病理损伤评估。