Animal Resource and Pathology Core

动物资源和病理学核心

• 批准号: 7514465
• 负责人: JAMES G FOX
• 金额: $ 20.65万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7514465
• 关键词: Animal Husbandry; Animals; Caring; Comparative Pathology; Containment; Controlled Environment; Embryo Transfer; Ensure; Equipment; Gene Silencing; Germ-Free; Hazardous Chemicals; Histology; Histopathology; Housing; Human Resources; Immunohistochemistry; Institutes; Laboratories; Medicine; Microinjections; Molecular; Mouse Strains; Nitrites; Pathogenesis; Pathology; Phenotype; Process; Program Research Project Grants; RNA Interference; Research; Research Personnel; Role; Safety; Services; Specific Pathogen Frees; System; Technology; Transgenic Animals; Transgenic Mice; Transgenic Organisms; Work; animal facility; animal resource; animal tissue; carcinogenesis; comparative; containment equipment; embryonic stem cell; germ free condition; in vivo; man; molecular pathology; mouse model; programs; square foot

Core 2 is essential for the delivery of optimal veterinary care and facilities utilized for the conduct of in vivo experimentation. The Pl.'s of this program project will have access to the Division of Comparative Medicine's AAALAC accredited, centrally managed and operated 175,000 sq. ft. of space, located in five buildings at the Institute. Each of the units has been built or extensively renovated in the last eight years. Four of these buildings have three corridor systems and three have specialized bio-containment units for infectious or hazardous chemical experimentation. Barrier facilities for housing specific pathogen free transgenic mouse colonies are also available. These animal facilities provide MIT investigators a controlled environment with the necessary space, equipment, and containment facilities for performing biomedical related research in animals including germ-free work and also ensures personnel safety during the experimental process. The DCM Comparative Pathology Laboratory will also provide the personnel and expertise to perform histological and immunocytochemical analysis of animal tissues delivered from in vivo studies performed within the framework of the program project. Specifically the role of Core 2 is to provide facilities, equipment, and personnel with expertise to centrally manage and supervise animal husbandry and experimental animal manipulations and provide histopathologic and transgenic services involved in studies conducted in the Program Project Grant. The specific aims are as follows: Specific Aim #1 To provide histology, immunohistochemistry, pathology and molecular characterization expertise for extensive phenotyping and assessment of pathological damage of mouse models used in this program Specific Aim #2 To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen free status of GEM used in this program Specific Aim #3 Provide colony management of mouse models for use by program project Pi's and as needed generate transgenic animals by pronuclear microinjection of DMAconstructs, embryonic stem cell manipulation, or gene silencing using RNAi technology.

核心2对于提供最佳兽医护理和用于进行体内 实验Pl.该计划项目的参与者将可以访问比较医学部的 AAALAC认证，集中管理和运营175，000平方米。英尺空间，位于五个建筑物在 院每一个单位都是在过去八年中建造或大规模翻修的。其中四 建筑物有三个走廊系统，三个有专门的生物遏制单位， 危险化学实验无特定病原体转基因小鼠的圈养屏障设施 也有殖民地。这些动物设施为麻省理工学院的研究人员提供了一个可控的环境， 进行生物医学相关研究所需的空间、设备和密封设施， 动物包括无菌工作，也确保了实验过程中的人员安全。的 DCM比较病理学实验室还将提供人员和专业知识， 和免疫细胞化学分析从体内研究中递送的动物组织， 方案项目的框架。具体来说，核心2的作用是提供设施、设备和 畜牧业和实验动物专业技术人员集中管理和监督 操作，并提供组织病理学和转基因服务，参与在 计划项目补助金。具体目标如下： 具体目标#1提供组织学、免疫组织化学、病理学和分子表征 专门知识用于小鼠广泛表型和病理损伤评估 本程序中使用的模型 具体目标#2通过胚胎移植重新衍生转基因和其他特化小鼠品系，以确保 本项目中使用的GEM无特定病原体状态 具体目标#3提供小鼠模型的群体管理，供项目Pi's使用， 需要通过DMA构建体的原核显微注射产生转基因动物， 胚胎干细胞操作或使用RNAi技术的基因沉默。