CORE--ISLET PRODUCTION

描述（由申请人提供）： 这个核心的主要目标是生产和提供，UCSF DRTC社区， 来自小鼠、非人灵长类动物（NHP）和人供体的胰腺组织和纯化胰岛 胰腺我们的重点是为每位研究者提供最好的胰腺组织和胰岛，确保这些样本的质量和可重复性。我们将专注于开发旨在最大限度地减少胰岛细胞死亡，最大限度地提高胰岛产量和优化胰岛功能的新技术。我们将与胰岛、b细胞领域的专家密切合作，以确定有助于我们实现更好的新胰岛分离技术目标的因素。该中心将继续开发检测方法，以更好地评估胰岛制剂的质量。研究更多的信息质量控制检测，这将前瞻性地预测体内胰岛功能，而不是依赖于今天有限的前瞻性研究。 核心将与基础科学家合作，我们提供胰腺组织和胰岛，以帮助我们充分利用胰岛细胞扩增和新生的新研究进入大规模胰岛移植环境。核心还将维持最先进的人类胰岛隔离设施 符合FDA关于人胰岛分离和移植的cGMP规定。核心还将维护NHP和啮齿动物胰岛隔离设施。核心将侧重于采取积极的方面，已经在人类胰岛分离实验室开发的，并将这些技术和测定应用于NHP和啮齿动物设施，反之亦然。胰岛生产设施核心将在DRTC的实验和临床项目中发挥关键作用，我们的目标是提供最好的组织，并帮助推进胰岛和β细胞研究。

DESCRIPTION (provided by applicant): The major objectives of this core are to produce and provide, to the UCSF DRTC community, pancreatic tissues and purified islets from mouse, non-human primates (NHP), and human donor pancreas. Our focus is to provide each investigator the best possible pancreatic tissue and islets, ensuring the quality and reproducibility of these samples. We will focus on developing new techniques that aim to minimize islet cell death, maximize islet yields and optimize islet function. We will work closely with experts in the islet, b-cell field to identify factors that will contribute to us reaching our goals for better new islet isolation techniques. The Core will continue to develop assays to better assess the quality of a pancreatic islet preparation. Investigate more informative quality control assays, which will prospectively predict in vivo islet function rather than the limited prospective studies relied upon today. The Core will work with the basic scientists, which we provide pancreas tissue and islets, to help us take full advantage of new research in islet cell expansion and neogenesis into large-scale islet transplantation settings. The Core will also maintain the state-of-the-art human islet isolation facilities meeting all FDA's cGMP regulations for human islet isolation and transplantation. The Core will also maintain the NHP and rodent pancreatic islet isolation facilities. The Core will focus on taking the positive aspects to what has been developed in the human islet isolation laboratory and apply these techniques and assays to both the NHP and rodent facilities and vise versa. The Islet Production Facility Core will play a critical role in the experimental and clinical programs of the DRTC and our objective is to provide the best possible tissues and to aid in the advancement of islet and beta-cells research.