Flow Cytometry

流式细胞仪核心将满足计划项目成员的特殊需求。的 高质量的分类和分析能力的可用性对于研究的成功至关重要 在这个项目中提出的努力补助金。我们将成为MoFlo细胞的活跃用户 分选和LSR II荧光分析仪中处理的研究： 基于细胞表面或转导基因标记的细胞;以及多参数分析， 检测罕见的淋巴细胞群体以及整体的生化和生物学变化 用各种基因转导的群体。在许多情况下，个别项目主任将 需要使用机器，这将需要三重甚至四重染色的复杂 在提案中列出的实验。该核心的具体目标是1。提供高速 细胞分选以分离可用于功能和生物化学研究的细胞; 2.提供 使用最先进的LSR II进行多参数分析的分析流式细胞术， 3.提供利用流式显微镜研究免疫耐受的创新方法。的 核心的可用性确保PPG研究者能够访问这一重要的 加州大学旧金山分校校园内的设施。

The Flow Cytometry Core will meet the special needs of the Program Project members. The availability of quality sorting and analysis capabilities is essential for the success of the research efforts proposed in this Program Project Grant. We will be active users of both the MoFlo cell sorting and LSR II fluorescence analyzer in the studies dealing with: sorting of small numbers of cells based on either cell surface or transduced gene markers; and multiparameter analyses to detect biochemical and biological changes in rare populations of lymphocytes as well as whole populations transduced with various genes. In many cases, the individual program directors will need access to machines that will require triple or even quadruple staining for the sophisticated experiments outlined in the proposal. The specific aims of this core are 1. To provide high speed cell sorting to isolate cells that can be used for functional and biochemical studies; 2. To provide analytical flow cytometry using a state-of-the-art LSR II to perform multiparameter analyses and 3. To provide innovative approaches to study immune tolerance using flow microscopy. The availability of the core ensures that the PPG investigators will have access to this essential facility on the UCSF campus.